Ines Kussmann

Review of: Ines Kussmann

Reviewed by:
Rating:
5
On 29.08.2020
Last modified:29.08.2020

Summary:

Ereignisse von Wolfgang Bahro alias Bommel des Adels eine besondere Vorkehrungen getroffen ist, fickt sie wgen nicht wissen. In diesen Geburtstag mit der 1. Bundesliga Abo zu knnen, mssen wir ehrlich gesagt, dass aufgrund seiner Fabrik an Felix von den Wondershare YouTube haben nur im Netz.

Ines Kussmann

Ines Kussmann is on Facebook. Join Facebook to connect with Ines Kussmann and others you may know. Facebook gives people the power to share and. Eine Behandlung mithilfe Arthrose Behandlung: Eigenbluttherapie in der Einen Pfau hat Tierärztin Ines Kussmann nur selten in der Behandlung. Da muss bei. Die Besitzer haben große Angst "Nola" zu verlieren und hoffen Tierärztin Ines Kussmann kann helfen. In der Untersuchung fällt der Tierärztin auf, dass die junge.

Ines Kussmann Treffer im Web

Als neue Mitinhaberin ist Frau Ines Kussmann seit Oktober in unser Team der Kleintierpraxis gekommen. Um uns fachlich noch breiter aufzustellen, haben​. Ines Kussmann ist bei Facebook. Tritt Facebook bei, um dich mit Ines Kussmann und anderen Nutzern, die du kennst, zu vernetzen. Facebook gibt Menschen. Ines Kussmann is on Facebook. Join Facebook to connect with Ines Kussmann and others you may know. Facebook gives people the power to share and. Ines Kussmann (Tierärztin) in Brookdeich 14, Hamburg ✓ Das sagen Nutzer über Frau Kussmann ✓ Finden Sie mehr zu Frau. Kleintierspezialisten, Bergedorf, Dr. Ulrike Ewe und Ines Kussmann in Hamburg ➤ Gemeinschaftspraxis ✓ Erfahrungsberichte echter. Ines Kussmann (Heimtiere, Zahnheilkunde, Chirurgie); Dr. Melanie Langer (​Zahnheilkunde, Ambulanz); Uta Rönneburg; Dr. Friedrich Müller (Klinikleiter. Impressum – Kleintierspezialisten Bergedorf – Praxis Dr. Ulrike Ewe und Ines Kussmann. Dr. med. vet. Ulrike Ewe und Frau Ines Kussmann Brookdeich

Ines Kussmann

Gutartig oder böse – das ist die alles entscheidende Frage. Die Tierärztinnen Dr. Melanie Langer und Ines Kussmann sollen den Patienten operieren. Bange. Kleintierspezialisten, Bergedorf, Dr. Ulrike Ewe und Ines Kussmann in Hamburg ➤ Gemeinschaftspraxis ✓ Erfahrungsberichte echter. Tierärztin Ines Kussmann Zunächst besteht für Tierärztin Ines Kussmann der Verdacht, dass sich der 21 Jahre alte Vogel beim Fliegen verletzt hat. Sieh dir an, was Ines Kussmann (ineskussmann) auf Pinterest entdeckt hat – die weltweit größte Ideensammlung. Ines-Kussmann«in Facebook - Finden Sie alle Informationen ✓ zur Person im sozialen Netzwerk. Aufnahmeort: Lüneburger Heide. Kamera: Lumix G6. Fotograf: Tierärzte Ines Kussmann Uwe Zimmermann Wittorf. Ines Kussmann/Dr. Uwe Zimmermann. Dr. Ulrike Ewe & TÄin Ines Kussmann. Dr. Mirja Fenske. Kleintiere. Osterrade 36a Hamburg Bezirk: Bergedorf Stadtteil: Lohbrügge. Tel: 10 40 Tierärztin Ines Kussmann staunt nicht schlecht, als sie plötzlich sechs Yorkshire-​Terrier im Behandlungszimmer empfängt. Doch wer von der Rasselbande ist. Ines Kussmann

Chemical Research in Toxicology , 33 2 , Chemical Research in Toxicology , 30 12 , Analytical Chemistry , 89 21 , Peterson, Pedro P.

Corbi, and Nicholas P. Inorganic Chemistry , 56 20 , Journal of Proteome Research , 16 9 , Ryan Parker, Jennifer S.

Journal of the American Society for Mass Spectrometry , 27 8 , Analytical Chemistry , 88 12 , Journal of Proteome Research , 15 5 , Analytical Chemistry , 87 22 , Bachschmid, Catherine E.

Costello, and Cheng Lin. Analytical Chemistry , 87 11 , Analytical Chemistry , 86 16 , Dykstra, Lois St.

Brice, Miguel Rodriguez, Jr. Cook, Lee R. Lynd, and Robert L. Journal of Proteome Research , 13 2 , Journal of Proteome Research , 12 11 , Hudson, Stephen E.

Halford, R. Leo Brady, and Derek N. Journal of the American Chemical Society , 34 , Dykstra, Miguel Rodriguez, Jr. Cook, and Robert L.

Analytical Chemistry , 85 6 , Pace, Douglas R. Brown, and Eranthie Weerapana. Journal of the American Chemical Society , 7 , Journal of Proteome Research , 11 11 , Harrington, Hao Chen.

Journal of the American Society for Mass Spectrometry , 23 3 , Rebecchi, Eden P. Go, Li Xu, Carrie L. Woodin, Minae Mure, and Heather Desaire.

Analytical Chemistry , 83 22 , Cabral, Nelson C. Journal of Proteome Research , 10 8 , Pietsch, and Shana J. Chemical Research in Toxicology , 24 5 , Moore, Tony Ly, and Ryan R.

Journal of the American Chemical Society , 18 , Zhang, V. Faca, and S. Journal of Proteome Research , 10 1 , Population Proteomics of the European Hake Merluccius merluccius.

Journal of Proteome Research , 9 12 , Journal of Proteome Research , 9 6 , Journal of Proteome Research , 9 5 , Soares, Maria P.

Journal of Proteome Research , 9 4 , Fernandez-Lima, David H. Amino acid influence on copper binding to peptides: Cysteine versus arginine.

Journal of the American Society for Mass Spectrometry , 21 4 , Journal of Agricultural and Food Chemistry , 58 4 , Journal of Proteome Research , 8 12 , Keith Williams, Daniel L.

Comins, Jerry L. Whitten, David C. Journal of the American Society for Mass Spectrometry , 20 11 , Journal of Agricultural and Food Chemistry , 57 9 , Analytical Chemistry , 80 9 , Keith Williams, Jr.

Meadows,, Ibrahim D. Bori,, Adam M. Hawkridge,, Daniel L. Comins, and, David C. Differential labeling of free and disulfide-bound thiol functions in proteins.

Journal of the American Society for Mass Spectrometry , 19 1 , Journal of Proteome Research , 7 1 , Froelich, Gavin E. Mechanisms for the proton mobility-dependent gas-phase fragmentation reactions of S-alkyl cysteine sulfoxide-containing peptide ions.

Journal of the American Society for Mass Spectrometry , 18 9 , Biochemistry , 46 32 , Accurate Mass Measurements in Proteomics.

Chemical Reviews , 8 , Kuipers and, Harry Gruppen. Journal of Agricultural and Food Chemistry , 55 14 , Frahm,, Ibrahim D.

Bori,, Daniel L. Comins,, Adam M. Hawkridge, and, David C. Analytical Chemistry , 79 11 , Analytical Chemistry , 79 7 , Chowdhury, Gerhard R.

Munske, Robert C. Ronald, James E. Journal of the American Society for Mass Spectrometry , 18 3 , Journal of Proteome Research , 5 8 , Analytical Chemistry , 78 13 , Journal of Proteome Research , 5 6 , Analytical Chemistry , 77 23 , Scott, Christopher C.

Benz, Michael A. Reactivity of zinc finger cysteines: Chemical modifications within labile zinc fingers in estrogen receptor.

Journal of the American Society for Mass Spectrometry , 16 12 , Bauer and, Eberhard Krause. Biochemistry , 44 5 , Rossier, Henrik Jensen, Hubert H.

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry. Journal of the American Society for Mass Spectrometry , 15 12 , Inerowicz, and, Fred E.

Analytical Chemistry , 76 15 , Patrie,, Leah M. Miller, and, Neil L. Journal of Proteome Research , 3 4 , Journal of Proteome Research , 3 3 , Analytical Chemistry , 75 24 , Neil Turner, and, Richard G.

Analytical Chemistry , 75 11 , Analytical Chemistry , 75 9 , Ogorzalek Loo, and, Joseph A. Journal of Proteome Research , 2 2 , Analytical Chemistry , 74 19 , Ogorzalek Loo, Joseph A.

Loo, Ping Du, Tod Holler. In vivo labeling: A glimpse of the dynamic proteome and additional constraints for protein identification. Journal of the American Society for Mass Spectrometry , 13 7 , VerBerkmoes,, Jonathan L.

Bundy,, Loren Hauser,, Keiji G. Hettich, and, James L. Stephenson, Jr. Journal of Proteome Research , 1 3 , Kindy,, John A. Taraszka,, Fred E.

Regnier, and, David E. Analytical Chemistry , 74 5 , Morton Bradbury, and, Xian Chen. Analytical Chemistry , 73 20 , Analytical Chemistry , 73 11 , Müller,, P.

Schindler,, H. Towbin,, U. Wirth,, H. Voshol,, S. Hoving, and, M. Isotope-Tagged Cross-Linking Reagents. Analytical Chemistry , 73 9 , Mass Spectrometry in Proteomics.

Chemical Reviews , 2 , Analytical Chemistry , 72 11 , Chait, and, David Fenyö. Analytical Chemistry , 72 5 , Goodlett,, James E.

Bruce,, Gordon A. Smith, and, Ruedi Aebersold. Analytical Chemistry , 72 6 , Kuznetsova, Lev I. Levitsky, Mikhail A.

Pyatnitskiy, Irina Y. Ilina, Julia A. Bubis, Elizaveta M. Solovyeva, Victor G. Zgoda, Mikhail V. Gorshkov, Sergei A. Cysteine alkylation methods in shotgun proteomics and their possible effects on methionine residues.

Journal of Proteomics , , Quantitative N-glycoproteomics using stable isotopic diethyl labeling. Talanta , , Journal of Intensive Care Medicine , 35 11 , Foods , 9 9 , Proteomic characterization of extracellular vesicles produced by several wine yeast species.

Microbial Biotechnology , 13 5 , Effect of high pressure and pulsed electric field on denaturation and allergenicity of Pru p 3 protein from peach.

Food Chemistry , , Proteomic analysis of Fusobacterium nucleatum growth in biofilm versus planktonic state. Molecular Oral Microbiology , 35 4 , Kuznetsova, E.

Solovyeva, A. Kuzikov, M. Gorshkov, S. Beaudoin, Jenelle A. Patterson, Jorge D. Harvey Millar, Andrew D. Bioinformatic and experimental evidence for suicidal and catalytic plant THI4s.

Biochemical Journal , 11 , Saeij, Luis Miguel Ortega-Mora. Comparative tachyzoite proteome analyses among six Neospora caninum isolates with different virulence.

International Journal for Parasitology , 50 5 , Molecules , 25 7 , Minor, F. Blaine Hollinger, Adrienne L. Cells , 9 4 , Analytica Chimica Acta , , Foods , 9 2 , Site-specific protein modification by genetic encoded disulfide compatible thiols.

Chinese Chemical Letters , 31 1 , Agronomy , 10 1 , Modification of cysteine residues for mass spectrometry-based proteomic analysis: facts and artifacts.

Biomeditsinskaya Khimiya , 66 1 , Emerging biotechnological approaches with respect to tissue regeneration: from improving biomaterial incorporation to comprehensive omics monitoring.

Spears, Abil E. Szijj, James R. Baker, Vijay Chudasama. Use of pyridazinediones as extracellular cleavable linkers through reversible cysteine conjugation.

Chemical Communications , 55 98 , Hawkins, Richard D. Silcott, Gregg A. A rational approach to form disulfide linked mucin hydrogels.

Soft Matter , 15 47 , Early antibody response and clinical outcome in experimental canine leishmaniasis. International Journal of Molecular Sciences , 20 22 , Plants , 8 11 , Brain pyrimidine nucleotide synthesis and Alzheimer disease.

Aging , 11 19 , Protein carbonylation is a mediator in larvicidal mechanisms of Tabernaemontana cymosa ethanolic extract. Journal of King Saud University - Science , 31 4 , Oxidative stress and mitochondrial uncoupling protein 2 expression in hepatic steatosis induced by exposure to xenobiotic DDE and high fat diet in male Wistar rats.

Greaser, Chad M. Reducing Complexity? Wolters, Hjalmar P. Permentier, Barbara M. Bakker, Rainer Bischoff. Targeted Proteomics to Study Mitochondrial Biology.

Membrane protein carbonylation of Plasmodium falciparum infected erythrocytes under conditions of sickle cell trait and G6PD deficiency. Molecular and Biochemical Parasitology , , Purslow, Trang T.

Nguyen, Timothy K. Egner, Rochelle R. Dotas, Balabhadra Khatiwada, Vincenzo Venditti. Biophysical Journal , 10 , Comprehensive identification of peptides in tandem mass spectra using an efficient open search engine.

Nature Biotechnology , 36 11 , Missense mutations have unexpected consequences: The McArdle disease paradigm.

Human Mutation , 39 10 , Proteomic profile changes associated with diminished expression of T-cell intracellular antigens reveal a hormesis response.

Biochemical and Biophysical Research Communications , 4 , Chemistry - A European Journal , 24 42 , Proteomics of Disulphide and Cysteine Oxidoreduction.

Markey, Yuri A. Mirokhin, Dmitrii V. Tchekhovskoi, Tallat H. Bukhari, Stephen E. Lakbub, Joshua T. Shipman, Heather Desaire. Recent mass spectrometry-based techniques and considerations for disulfide bond characterization in proteins.

Analytical and Bioanalytical Chemistry , 10 , Current Protocols in Cell Biology , 78 1 , 5. Jetten, Rob J. Mesman, Laura van Niftrik.

Characterization of the first planctomycetal outer membrane protein identifies a channel in the outer membrane of the anammox bacterium Kuenenia stuttgartiensis.

Methods and Protocols , 1 1 , 2. Probing the Protein: Ion Mobility Spectrometry. Proteomes , 5 4 , A light-sensitive mutation in Arabidopsis LEW3 reveals the important role of N-glycosylation in root growth and development.

Journal of Experimental Botany , 68 18 , Biochemical characterization of argininosuccinate lyase from M. The KIM-family protein-tyrosine phosphatases use distinct reversible oxidation intermediates: Intramolecular or intermolecular disulfide bond formation.

Journal of Biological Chemistry , 21 , International Journal of Mass Spectrometry , , Angewandte Chemie , 16 , Angewandte Chemie International Edition , 56 16 , PLOS Biology , 15 4 , e Chemoselective Modification of Proteins.

Graham, Martin F. Lavin, Sergei V. Shabb, W. Muhonen, A. Evaluation and optimization of reduction and alkylation methods to maximize peptide identification with MS-based proteomics.

Molecular BioSystems , 13 12 , Barderas, Luis M. Urinary alpha-1 antitrypsin and CD59 glycoprotein predict albuminuria development in hypertensive patients under chronic renin-angiotensin system suppression.

Prieto, Maria Montoya. Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection.

Proteins adopt functionally active conformations after type III secretion. Redox regulation of ATP sulfurylase in microalgae.

Antimicrobial Agents and Chemotherapy , 60 7 , A method to identify protein antigens of Dermanyssus gallinae for the protection of birds from poultry mites.

Parasitology Research , 7 , New insights into the post-translational modification of multiple phosphoenolpyruvate carboxylase isoenzymes by phosphorylation and monoubiquitination during sorghum seed development and germination.

Journal of Experimental Botany , 67 11 , Substoichiometric inhibition of transthyretin misfolding by immune-targeting sparsely populated misfolding intermediates: a potential diagnostic and therapeutic for TTR amyloidoses.

Davies, EonSeon Jin,. Maroto, Paula J. Cytoskeleton deregulation and impairment in amino acids and energy metabolism in early atherosclerosis at aortic tissue with reflection in plasma.

Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis?.

Ion source-dependent performance of 4-vinylpyridine, iodoacetamide, and N-maleoyl derivatives for the detection of cysteine-containing peptides in complex proteomics.

Analytical and Bioanalytical Chemistry , 8 , Rogers, Ryan D. Selective chemical labeling of proteins. High production of ectoine from aspartate and glycerol by use of whole-cell biocatalysis in recombinant Escherichia coli.

Dewald, Rebecca Zhou, Hao Chen. The study of electrochemistry with ambient mass spectrometry. Immunoproteomic profiling of Saccharomyces cerevisiae systemic infection in a murine model.

Aeromonas hydrophila produces conductive nanowires. Research in Microbiology , 9 , Food Research International , 63 , Kilpatrick, Yuxue Liang, Yuri A.

Mirokhin, Jeri S. Roth, Paul A. Rudnick, Stephen E. Current Protocols in Protein Science , 77 1 , Arnold, Sean D. Laguna, P. Eigenvalue-based time delay estimation of repetitive biomedical signals.

Digital Signal Processing, 75 EMG activity during reaching and grasping. Pisa, Italy Honolulu, USA Rafols-De-Urquia, M. Assessment of respiratory muscle activity with surface.

Lozano-Garcia, M. Assessment of inspiratory muscle activation using surface diaphragm. Winfried J. KG, Karlsruhe, Germany Prof.

Giraldo, B. Respiratory sinus arrhythmia quantified with linear and non-linear techniques to classify dilated and ischemic cardiomyopathy.

A novel artifact reconstruction method applied to blood pressure signals. Cardiorespiratory phase synchronization increases during certain mental stimuli in healthy subjects.

In this context, we are interested in intelligent chemical instruments for the detection of volatile compounds and smells.

These systems can be based on an array of nonspecific chemical sensors with a pattern recognition engine, taking inspiration from the olfactory system.

Some spectrometries, e. Ion Mobility Spectrometry, are capable of very fast analysis with good detection limits but poor selectivity.

These technologies have been proposed for the fast determination of the volatolome volatile fraction of the metabolome , instead of the reference technique of gas chromatography — mass spectrometry.

Our Research in included 1. We have studied the impact of low power operation modes in the performance of Metal Oxide Sensors 4. We are developing algorithms for the analysis of mass spectrometry images for the study of heterogeneity in colorectal cancer tissues 5.

We are developing analytical methods for the study of the majoritary compounds in human flatus in collaboration with Dr.

We have proposed methods to estimate the limit of detection in non-linear chemical sensors using univariate and multivariate calibration models 7.

We have proposed methods to find universal calibration models for chemical sensor arrays 8. We have studied the impact of cross-validation methods in the overoptimism of PLS-DA models applied to omics data 9.

We have proposed methods to evaluate and correct the impact of instrumental shifts in GC-MS data for breath metabolomics.

Multiunit calibration rejects inherent device variability of chemical sensor arrays. Sensors and Actuators B: Chemical, Contreras, M. Thermal desorption-ion mobility spectrometry: A rapid sensor for the detection of cannabinoids and discrimination of Cannabis sativa L.

Sensors and Actuators B:. Chemical, Multivariate estimation of the limit of detection by orthogonal partial least squares in temperature-modulated MOX sensors.

Analytica Chimica Acta, Estimation of the limit of detection in semiconductor gas sensors through linearized calibration models.

Fernandez, L. A practical method to estimate the resolving power of a chemical sensor array: Application to feature selection.

Frontiers in Chemistry, 6 Article Oller-Moreno, S. The differential plasma proteome of obese and overweight individuals undergoing a nutritional weight loss and maintenance intervention.

Gardner, Microsensors and Bioelectronics Lab, Dept. Ivan Montoliu and Dra. Journal of Breath Research, 12 3 : Overoptimism in crossvalidation when using partial least squares-discriminant analysis for omics data: a systematic study.

Analytical and Bioanalytical Chemistry, 23 : Taghadomi-Saberi, S. Classification of bitter orange essential oils according to fruit ripening stage by untargeted chemical profiling and machine learning.

Sensors, 18 6 : Fonollosa, J. Chemical sensor systems and associated algorithms for fire detection: A review. Sensors, 18 2 : Low power operation of temperature-modulated metal oxide semiconductor gas sensors.

Graz, Austria The need of external validation for metabolomics predictive models. Cumeras, R. The main conceptual problem of the standard in vitro cell-based assays is that they rely on two dimensional monolayer cellular cultures, which fail to replicate the complexity of living systems.

There is an urgent need to create technological platforms with complex cell culture systems that mimic better the tissue-like cellular microenvironment.

We propose to combine engineering microfabrication technologies, tissue engineering concepts and recent advances in stem cell research, exploiting stem cell unique properties, to create cell culture microenvironments that will go beyond current 3D in vitro models.

Resulting in vitro tissue equivalents aim at. The cell culture platforms proposed will provide physiologically relevant and highly reproducible data, and they will be compatible with conventional cell culture assays and high-throughput testing.

The new organotypic cell culture platforms will aim to advance the in vitro modelling of diseases, the preclinical screening for drug toxicity, the understanding of organ development and the regenerative medicine applications.

Current main projects are: i to engineer and validate a complex in vitro model of small intestinal epithelium and ii to engineer and validate a novel in vitro model of engineered cardiac tissue.

Mimicking small intestinal tissue by culturing intestinal-organoid derived cells on poly ethylene glycol diacrylate PEGDA microstructures.

Cell nuclei are stained in green and actin cytoskeleton in red. The role of mucus as an invisible cloak to transepithelial drug delivery by nanoparticles.

Hortigüela, V. Nanopatterns of surface-bound ephrinB1 produce multivalent ligand-receptor interactions that tune EphB2 receptor clustering.

Nano Letters, 18 1 : Macedo, M. Derived enterocyte-like cells for drug absorption and metabolism studies. Trends in Molecular Medicine, 24 8 : Torras, N.

Mimicking epithelial tissues in threedimensional cell culture models. Javier Santos, Dra. Conference Papers de Goede, M. High quality factor Al2O3 microring resonators for on-chip sensing applications.

California, USA Al2O3 Mmicroresonators for passive and active sensing applications. Optical Sensors. Zurich, Switzerland Al2O3 Microresonator based passive and active biosensors.

Bucharest, Romania Successful replacement, or augmentation, of the function of damaged cells by patient derived differentiated stem cells would provide a novel cell-based therapy for diseases.

Since iPSCs resemble human embryonic stem cells hESCs in their ability to generate cells of three germ layers, patient-specific iPSCs offer definitive solutions for the ethical and histo-incompatibility issues related to hESCs.

One of our aims is to generate and correct diseasespecific hiPSCs for disease modelling and drug screening.

The combination of gene-editing based methodologies together with the development of novel. In this regard we are particularly interested in generation of transgene-free and disease free patient derived hiPSCs for disease modelling and the discovery of novel therapeutic targets.

We believe that the recovery of tissue function should not be restricted to the development of cell replacement therapies.

In this regard, in our laboratory we take advantage of organisms that possess the ability to regenerate such as zebrafish, in order to understand which molecular and cellular pathways lead to organ regeneration.

Surprisingly, studies in neonatal mice have demonstrated that soon after birth this organism posses the capability to regenerate its heart.

Taking advantage of such preliminary observations we are translating such analysis in order to understand if the mammalian neonatal kidney still posses the capability to regenerate, and more importantly, if we are able to dissect the epigenetic and cellular mechanisms leading to those responses.

Lastly, and in an effort to fully develop in vitro and ex vivo platforms for organ regeneration, in our lab we are focused in the development of reporter cell lines for different transcription factors essential for tissue-specific commitment and differentiation i.

The possibility to combine pluripotent stem cell lines together with decellularized matrices, functionalized biomaterials and ex vivo organoids offers and unprecedented opportunity for the immediate generation of patient-specific in vitro and ex vivo platforms for disease modelling and organ regeneration Figure 2.

Latorre, E. Active superelasticity in threedimensional epithelia of controlled shape. Nature, : Hernandez-Benitez, R. Martinez, M. At the heart of genome editing and cardiovascular diseases.

Circulation Research, 2 : Niederberger, C. Swain, J. Laufer, N. Forty years of IVF. Fertility and Sterility, 2 : Hurtado del Pozo, C.

Modeling epigenetic modifications in renal development and disease with organoids and genome editing. Garreta, E.

Studying kidney disease using tissue and genome engineering in human pluripotent stem cells. Nephron, Roadblocks in the path of iPSC to the vlinic.

Current Transplantation Reports, 5 1 : Kidney organoids for disease modeling. Oncotarget, 9 16 : However, despite such a great advance and promise, our ability to treat diseases such as neurological maladies, genetic syndromes and cancer, remains a major challenge.

One of the prime obstacles is. A plethora of promising tools are becoming available to tackle health problems, such as new drug carriers or delivery systems, macromolecular assemblies within the nanoscale-size range which can be loaded with diagnostic and therapeutic agents to improve their solubility,.

Pictures are reproduced or adapted from the following sources Copyrights reside on the respective publishers and associated professional societies : [1] Mane et al.

Our lab generates knowledge and tools aimed to improve our ability to deliver therapeutic agents to specific disease sites. Focusing on endothelial cell adhesion molecules as examples of accessible targets and on genetic conditions which serve as models for metabolic, neurodegenerative and cardiovascular syndromes, our ultimate goal is to enable effective treatment for these life-threatening disorders and other maladies characterized by similar pathological traits.

Some of our main programmatic efforts are described below. Biologically-Controlled Transport of Drug Carriers How drug delivery systems are sensed, transported, and disposed of within the body, which is greatly dependent upon biological properties and processes, is far from being understood and much less controlled.

Most targeted strategies are designed to achieve specific binding of drug delivery systems to cellsurface receptors, but then they simply depend on the signaling and transport processes the bound receptor regulates in nature.

Instead, by deciphering the biological bases of these events, we impart the drug carrier control over biological signaling events independently from the receptor being bound, bypassing.

J Control Release. This provides a new and complementary avenue at the interface between the use of novel technological tools to decipher the biological mechanisms that regulate health and fail in disease, and the use of biological knowledge to optimize nanotechnology tools aimed to diagnose and treat human pathologies.

For instance, we have shown how even using the same targeting or receptor, the kinetics, mechanism, and destination of a drug carrier can be modulated by: a varying its size, shape, and targeting valency; b varying the receptor epitope to which the carrier binds; c using auxiliary drugs to modulate the endocytic machinery; d coupling carriers to signaling molecules that can tune the uptake route independently from the receptor being used Figure 2 ; e combining targeting to several receptors; or f coupling targeting moieties with anti-phagocytic moieties on the surface of drug carriers.

Transport of Drug Carriers Across Physiological Barriers Crossing the linings that separate body and cellular compartments is paramount for efficient drug delivery.

For instance, an epithelial barrier separates the gastrointestinal tract from the bloodstream, controlling uptake of orally ingested substances.

While certain chemical entities are able to cross this barrier, many therapies do not and their successful utilization needs of means to bypass this obstacle.

As for neurodegenerative conditions, they remain largely untreatable because. Muro S. Another example is that of novel biological therapeutics, which have demonstrated potential to manipulate disease targets far more precisely than their small chemical counterparts.

However, these large and fragile therapeutics fail to traverse the membranes that separate the extracellular environment from the intracellular milieu and those of intracellular.

We demonstrated that the ICAM-1 pathway described in the next section enables transcytosis across epithelial and endothelial linings, which we explore for oral delivery and delivery across the bloodbrain barrier.

We were also able to target DNA-built dendrimers to cells specifically, whereby these DNA dendrimers enabled endosomal escape and cytosol delivery of a variety of cargoes, including small toxins, carbohydrates Figure 3 , proteins, and nucleic acids.

ICAMtargeted nano- and micro-carriers are both internalized by cells due to natural sphingomyelinase SMase -dependent generation of ceramide at ICAMbinding sites.

Ceramide improves carrier engulfment and membrane invagination, and acts as a second messenger toward actin re-organization, helping endocytic uptake left panel.

In contrast, targeting drug carriers to receptors associated with more size-restrictive pathways, e. Surface-functionalization of M6PR-targeted carriers with elements mimicking the ICAM-1 pathway, namely exogenous SMases such as NSM , supplies the necessary ceramide and actin re-organization, improving endocytosis of nano- and micro-carriers even when targeted to receptors different from ICAM-1 right panel.

Reproduced from Ansar et al. This new transport route is different from most others classically utilized for drug delivery, including clathrin-, caveolar-, macropinocytosis-, or phagocytosis-mediated pathways.

My independent laboratory continues to unravel the regulation of this route, particularly focusing on ICAM-1 Figure 4 , and its implications in patho-physiology and drug delivery.

The relevance of this new pathway is illustrated by the fact that ICAM-1 mediates extravasation of leukocytes during inflammation, signaling at the immune synapsis, and invasion by some pathogens e.

The understanding of this fundamental route and its properties is also advancing diverse drug delivery applications by our group and many others.

Improving Treatment of Lysosomal Disorders Monogenic pathologies due to genetic deficiency, such as the case of lysosomal disorders, are valuable models to study disease progression and therapeutic.

Also, their unequivocal diagnosis enables the tracing of their progression from early to late stages. Since these diseases present with either acute or long-term effects depending on genetic severity, and associate with neurodegeneration, cardiovascular, metabolic, and cancer-like syndromes, they represent excellent disease models.

The current lack of efficient therapies to treat these syndromes stems from problems similar to those described above, i.

Consequently, we are applying targeted nanotechnology concepts to the treatment of genetic lysosomal disorders. Current therapies by i. Yet, delivery to other organs brain, lungs, etc.

Using types A and B Niemann-Pick Figure 5 , Fabry, and Gaucher diseases as examples, we have shown improved delivery of therapeutic enzymes to all affected organs in animal models, holding considerable translational potential.

Left Illustrative cartoon and corresponding microscopy showing that fluorescent dextran delivered to cells via targeted polymer nanoparticles resides in vesicular compartments bright red spots around the cell nucleus blue.

DNA-built dendrimers. Adapted from Muro Adv Funct Mat, 24 19 Adapted from Serrano et al. Top Polymer nanocarriers NCs bearing therapeutic acid sphingomyelinase ASM and targeted to ICAM-1 were observed by fluorescent microscopy to abundantly reach the lungs, as observed 30 min after i.

Bottom Transmission electron microscopy of lungs collected 3 h after i. For instance, NCs can be seen being engulfed by cells black arrows , within cell endosomes white arrowheads and lysosomes black arrowheads , and transcytosed across the endothelium into subjacent epithelial cells white arrow.

Reproduced from Garnacho et al. Juan Marugan and Dr. Brigitte Städler, Aarhus University, Denmark. Biosafety level 2 culture hoods CO2 incubators Liquid nitrogen storage tank Thermocycler Confocal-epifluorescence microscope Spectrofluorometer.

Cellular and respiratory biomechanics Daniel Navajas The goal of our research is to gain a deeper understanding of cellular and respiratory biomechanics to improve the diagnosis and treatment of respiratory diseases.

The work is organized in two interrelated areas, focused on respiratory mechanics at both the systemic and the cellular level. We use basic and translational approaches in a multidisciplinary framework involving close cooperation with clinical groups.

Our current research interest is focused on the study of cell-matrix mechanical cross-talk for tissue engineering and regenerative medicine.

Cells sense and actively respond to the biophysical features of their microenvironment. Mechanical properties of the extracellular matrix regulate critical cell processes such as contraction, migration, proliferation, gene expression and differentiation.

We use atomic force microscopy and other cutting-edge biophysical techniques to study the mechanical properties of the extracellular matrix and their impact in cell behavior.

We have implemented protocols to decellularize different soft tissues. This innovative. By seeding cells in these scaffolds we study the impact of the mechanical features of the microenvironment on stem cell engraftment and differentiation onto lung and heart phenotypes.

We produce lab-on-chip devices mimicking the native cell microenvironment to investigate mechanical signaling driving stem cell differentiation under precisely controlled conditions.

Using 3D bioprinters we integrate stem cells into synthetic and extracellular matrix hydrogels to fabricate tissue patches as an innovative approach to regenerate ventricular scars resulting from heart infarct.

Organ biofabrication reengineered from decellularized tissue scaffolds offers a promising alternative for transplantation.

We develop improved bioreactors mimicking breathing and blood perfusion to biofabricate lungs by seeding stem cells into acellular lung scaffolds.

Villanueva, J. A portable continuous positive airway pressure device that can perform optimally under strenuous conditions.

Torres, M. Aging reduces intermittent hypoxiainduced lung carcinoma growth in a mouse model of sleep apnea.

Is telemedicine a key tool for improving continuous positive airway pressure adherence in patients with sleep apnea? Notari, M. The local microenvironment limits the regenerative potential of the mouse neonatal heart.

Science Advances, 4 5 : eaao Alcaraz, J. Bidirectional mechanobiology between cells and their local extracellular matrix probed by atomic force microscopy.

Seminars in Cell and Developmental Biology, Perea-Gil, I. Head-to-head comparison of two engineered cardiac grafts for myocardial.

Menal, M. Frontiers in Neurology, 9: Article 1 Intermittent hypoxia mimicking sleep apnea increases passive stiffness of myocardial extracellular matrix.

A multiscale study. Frontiers in Physiology, 9: Article Passive stiffness of left ventricular myocardial tissue is reduced by ovariectomy in a post-menopause mouse model.

Bioengineered lungs: A challenge and an opportunity. In the case of animal models we have not only some ethical problems but also the ability to extrapolate data to human conditions is limited and in vitro platforms often do not simulate the complex cell—cell and cell—matrix interactions crucial for regulating cell behaviour.

Biosensors for bioengineering group is focused in a new line of research that has become of extreme importance in the last years.

The idea is to integrate biosensor technology and nanotechnology with stem cell research and with tissue engineering.

Engineered tissues are integrated with biosensing technology to obtain microdevices for detecting cellular responses to external stimuli, monitoring the quality of the microenvironment e.

This research on 3D-functional engineered tissues is expected to develop knowledge of tissue construction and their functions and relation with some human diseases.

These chips could be used in pharmaceutical assays and could be a step toward the ultimate goal of producing in vitro drug testing systems crucial to the medicine and pharmaceutical industry.

Pancreas islet stained in blue for nuclei and red for actin inside a microporous microfibrillated cellulose-gelatin scaffold stained with green.

Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissue. Macromolecular Bioscience, 18 10 : Conference Paper.

We believe that combining several different stimuli in the chip resembles a more realistic environment that nerve cells will encounter in the living animal in normal and disease conditions.

These experiments using Labon-a-Chip models in turn will make future studies on the role of neuronal cells in development and regeneration more accurate and complete.

Thus, we developed a new device able to reproduce the formation of the neuromuscular junction NMJ in lab on chip devices.

In addition, a device designed to analyse axon lesions of cortical neurons was also developed. Current experiments.

For example: i on chip lab platform to monitor drug delivery modulating neuronal activity see figure ; ii cortico-spinal chips to develop axon regenerative studies of new drug formulation in collaboration with Imperial College; London ; iii devices to molecular gradient generation for migrating neurons in collaboration with i3A, Zaragoza and, iv in silico 3D modeling for neurodegenerative diseases Alzheimer and Parkinson chip.

Last experiments are focused to reproduce a lab on chip devices to analyse and to reproduce ALS models in collaboration with Biodonostia Hospital Figure 2.

However, the basis of the spreading process remains poorly understood although cell to cell transport via. Experimental evidence suggests that providing a permissive extracellular environment containing cellular or biomaterial bridges is not enough to increase axon regrow to obtain functional regeneration, supporting the importance of clarifying the complex transcriptional response below these signalling pathways.

As an alternative, activating or recapitulating the developmental program of the lesioned neuron to. Hervera, A. Nature Cell Biology, 20 3 : Role of cellular prion protein in interneuronal amyloid transmission.

Progress in Neurobiology, Urrea, L. Molecular Neurobiology, 55 3 : Optogenetics is currently the state-of-the-art method for activity-based nervous system research, allowing more specific cellular stimulations by light in a less invasive techniques compared to classical electrical stimulation, but leading to a more tailored physiological responses.

In our laboratory, we started to analyse whether optical stimulation of lesioned neurons is able to enhance axonal growth or sprouting.

These experiments were performed on lesioned cortical neurons and in vivo. Matamoros-Angles, A. Molecular Neurobiology, 55 4 : Llorens, F.

Vivancos, A. Regional and subtype-dependent miRNA signatures in sporadic Creutzfeldt-Jakob disease are accompanied by alterations in miRNA silencing machinery and biogenesis.

PLoS Pathogens, 14 1 : e Garcia-Esparcia, P. Brain Pathology, 28 1 : Ferrer, I. Brain Pathology, 28 6 : — Elisabeth Engel page 13 , Prof.

Josep Samitier, page 61 , Prof. Xavier Trepat, page 75 and Prof. Antonella Consiglio and Dr. Beth L.

Mata, A. New functions of Semaphorin 3E and its receptor PlexinD1 during developing and adult hippocampal formation. Franco, R. N-methyl-D-aspartate receptor link to the MAP kinase pathway.

Frontiers in Molecular Neuroscience, 11 : Article Tomas-Roig, J. Effects of repeated. Badiola-Mateos, M. Challenges and future prospects on 3D in-vitro modeling of the neuromuscular circuit.

Frontiers in Bioengineering and Biotechnology, 6 Article Cellular and molecular mechanobiology Pere Roca-Cusachs Every time we blink, move a hand, draw a breath, or walk, cells in our body exert, transmit, withstand, and detect forces.

This mechanical interaction with the environment determines how cells proliferate, differentiate, and move, and regulates development, tumorigenesis or wound healing.

Just like biochemical stimuli initiate signaling cascades, mechanical forces affect the links and conformation of a network of molecules connecting cells to the extracellular matrix.

Our research aims precisely at unraveling the mechanisms that these molecules use to detect and respond to mechanical stimuli like forces or tissue rigidity, triggering downstream cell responses.

To this end, we combine biophysical techniques like magnetic tweezers, Atomic Force Microscopy, traction microscopy, and microfabricated force sensors with molecular biology, advanced optical microscopy, and theoretical modelling.

Sensing rigidity: Using this multi-disciplinary approach, we have recently unveiled a molecular mechanism that cells employ to detect and respond to the rigidity of their environment, which could be crucial in breast tissue.

Cell Biol. This affects force transmission from the environment to cells, and also within different cell components.

Recently, we have begun to explore how force transmission to the nucleus affects the dynamics of transcriptional regulators, such as YAP Elosegui-Artola et al.

Sensing the environment: We are currently expanding on the idea of the molecular clutch, to explore how cell molecular engines sense not only mechanical rigidity, but other important parameters from their environment: for instance, the composition and distribution of ligands.

Artistic rendering of a cell attaching to a substrate coated with a gold nano-pattern array, used to study how cells detect spatial cues From Oria et al.

In this regard, we recently uncovered that this concept can explain how cells sense the spatial distribution of ligands in the extracellular matrix Oria et al.

We have also demonstrated that cell-cell force transmission, mediated by a molecular clutch, is essential for cells to sense gradients in stiffness Sunyer et al.

Ultimately, when we determine the molecular mechanisms that communicate cells with their environment, we will understand how forces determine development when things go right, and tumor formation when they go wrong.

The membrane as a mechanosensor: Due to its mechanical properties, the plasma membrane itself can respond to forces and act as a mechanosensor.

Recently, we have shown that cell membranes can use purely physical principles to adapt their shape in response to mechanical forces Kosmalska et al.

We are currently studying how cells harness this physical membrane behavior to respond to signals from their environment.

Cartoon depicting how force transmission to the nucleus affects nuclear pores, leading to nuclear protein import from Elosegui-Artola et al.

Elosegui-Artola, A. Control of. Trends in Cell Biology, 28 5 : Thottacherry, J. Mechanochemical feedback and control of endocytosis and membrane tension.

Nature Communications, 9 Gauthier, N. Mechanosensing at integrin-mediated cell—matrix adhesions: from molecular to. Current Opinion in Cell Biology, 50 Bennett, M.

Molecular clutch drives cell response to surface viscosity. Roca-Cusachs, P. Cell scientist to watch - Pere Roca-Cusachs.

Journal of Cell Science, 16 : jcs Escribano, J. A hybrid computational model for collective cell durotaxis. Biomechanics and Modeling in Mechanobiology, 17 4 : Nanobioengineering Josep Samitier The Nanobioengineering group is a truly multidisciplinary team composed by researchers coming from very diverse backgrounds working together in applying nanotechnology for the development of new biomedical systems and devices, mainly for diagnostic purposes, and integrated microfluidic Organ-on-aChip devices for the study of organ physiology, disease etiology, or drug screening.

The main research activities of the group include the engineering and biochemical functionalization of biomaterials integrated with microfluidics systems.

The bioengineered microdevices are used to study cell responses to biomolecular compounds applied to Organon-Chip devices, or for the development of new Lab-on-aChip based biosensors.

The goal is to fabricate microsystems containing living cells that recapitulate tissue and organ level functions in vitro and new portable diagnosis devices that can be used as Point-of-Care systems.

The projects carried out by the group are focused on clinical and industrial problems and are related to three convergent research lines:.

Tumor Cancer on a chip in vitro development Microfluidic Vessel on-a-chip for screening drug delivery systems. Katuri, J. Directed flow of micromotors through alignment interactions with micropatterned ratchets.

ACS Nano, 12 7 : Gorostiza, P. Molecular architecture for DNA wiring. Biosensors and Bioelectronics, Blood-based cancer biomarkers in liquid biopsy: A.

U nanoET-leukemia Nanoconductance of electron transfer proteins of the respiratory chain. International Journal of Molecular Sciences, 19 10 : Silva, N.

Nanomaterials, 8 12 : Cross-fertilization of Key Enabling Technologies: An empirical study of nanotechnology-related projects. Dana-Farber Cancer Institute, Inc.

Journal of Engineering and Technology Management, 49 Multi-disciplinarity breeds diversity: the influence of innovation project characteristics on diversity creation in nanotechnology.

Journal of Technology Transfer, 43 2 : Juan C. Producing 3D biomimetic nanomaterials for musculoskeletal system regeneration. Sampietro, Politecnico di Milano, Italy Prof.

Molly M. Miguel A. Industry partners: Biokit S. Werfen group ; Genomica S. Zeltia group ; Tallers Fiestas S. We are interested in fundamental studies of active matter, the use of nanobots for future nanomedicine and environmental applications and the bioengineering of new devices based on hybrid systems.

Nano-Bio Team The use of enzyme catalysis is emerging as an attractive alternative to power micro- and nanomachines due to their unique features including biocompatibility, versatility and fuel bioavailability.

Our group has demonstrated the use of different enzymes, including urease and glucose oxidase, to generate active propulsion of nano- and microparticles, paving the way towards new applications of artificial active matter in biomedicine.

In particular, we take advantage of the 3D bioprinting technique to develop bio-robotic systems composed of skeletal muscle cells embedded. Smart micro- and nanorobots are able to swim, monitor their own activity, sense their environment and deliver drugs to 3D bladder cancer spheroids using biocompatible and bioavailable fuels such as urea.

These materials can act as scaffolds, support, or flexible parts, as well as be responsive upon certain stimuli. By controlling the contractions of skeletal muscle cells via electric fields, we can measure the forces exerted by these bio-actuators against artificial 3D-printed posts.

Using this setup, we have performed studies on the adaptability of bio-actuators after applying different training protocols and we have observed how their force generation and gene expression can adapt to the frequency of stimulation and stiffness of the artificial posts.

Active matter in complex systems We study colloidal suspensions of Pt-coated silica particles as a model system of synthetic active matter.

These systems have mostly been studied in homogeneous environments until now. Our interest lies in observing these systems in more complex settings, such as near interfaces, complex media or with flow involved.

Since the self-propelled particles generate chemical and hydrodynamic fields around them, they interact in complex ways with flows and nearby surfaces that often leads to.

Upon electrical stimulation, the muscle can contract, bend the post and their force can be calculated. Fundamental aspects of enzyme-powered micro- and nanoswimmers.

Accounts of Chemical Research, 51 11 : — Influence of enzyme quantity and distribution on the self-propulsion of non-Janus urease-powered micromotors.

Journal of the American Chemical Society, 30 : Vilela, D. Medical imaging for the tracking of micromotors. When flow is present, particles also behave different as they reorient perpendicular to the flow.

Environmental applications of micro-nano motors Artificial micromotors, based on bubble self-propulsion have demonstrated to be able to mix solutions and.

These micromotors are mostly based on two main structures, tubular and spherical. On the other hand, electrodeposited microjets, which are smaller.

Catalytically active colloids generate chemical gradients and interact with their nearby surfaces while they swim.

When they swim in capillaries where a flow is imposed, they present a cross stream migration deviating from straight trajectories, perpendicular to the flow lines.

This type of motion resembles, in some aspects, the motion of biological microswimmers. ACS Nano, 12 2 : Wang, X. Fuel-free nanocap-like motors actuated under.

Advanced Functional Materials, 28 25 : Enzyme-powered nanobots enhance anticancer drug delivery. Advanced Functional Materials, 28 Xuan, M.

Noncontinuous super-diffusive dynamics of a light-activated nanobottle motor. Angewandte Chemie International Edition, 57 23 : Cross-stream migration of active particles.

Science Advances, 4 1 : eaao In this case, the metal is adsorbed and removed from the contaminated water. The metal can thereafter be desorbed and the microjets used again.

In order to target other water pollution problems, such as microorganism contamination, we have developed spherical microbots that can kill bacteria while they swim.

These microbots have a Janus structure based on spherical magnesium microparticles, able to dissolve in water producing hydrogen bubbles, covered in one of their faces by Fe, Au and AgNPs which provide magnetic, bacteria attachment and bactericidal properties to the microjets.

Towards scaling-up of the micromotor synthesis for cleaning large volumes of water, we have fabricated micromotors using exclusively chemical methods such as, precipitation, reduction and sol-gel chemistry.

These micromotors are based on a silica microtubular structure which contains an inner-layer of a catalytic material PtNPs or MnO2 capable of removing pollutants efficiently from water while they swim in the presence of hydrogen peroxide.

The external decoration of these structures with magnetic nanoparticles provides for good magnetic control. Finally, magnetic and catalytic micromotors formed by the aggregation of cobalt ferrite nanoparticles were synthesized to remove antibiotics from water.

All these micromotors, due to their magnetic properties can be removed from the solution after finishing their targeting action by the application of an external magnetic field.

Villa, K. Metal-oxide-based microjets for the simultaneous removal of organic pollutants and heavy metals. Romeo, A. Inkjet printed flexible non-enzymatic glucose sensor for tear fluid analysis.

Applied Materials Today, 10 Core-shell microspheres for the ultrafast degradation of estrogen hormone at neutral pH. RSC Advances, 8 11 : Conference Papers Mestre, R.

Paris, France Point-of-care diagnostics allows decentralizing clinical diagnostic practices and monitoring health out of specialized hospital settings.

Advantages of such decentralization are improved quality of life of patients, enhanced therapeutic efficacy thanks to more frequent tests, and lower overall cost of the health system.

We develop flexible biochemical sensors for non-invasive and cost-effective monitoring of analytes in biological fluids alternative to blood, e.

We combine electrochemical electrochemical sensors with microfluidics and electronics to achieve fully integrated devices, that are well suited for low-cost, portable and user-friendly medical diagnostics.

Dietrich, Dr. Popescu, M. Tasinkevych, Dr. Analytes are quickly quantified by electrochemical techniques. Gabriel and Prof. Bacterial Infections: Antimicrobial Therapies Eduard Torrents Infectious diseases constitute a tenacious and major public health problem all over the world.

The emergence and increasing prevalence of bacterial strains that are resistant to available antibiotics demand the discovery of new therapeutic approaches.

There is an urgent need for reliable and rapid detection of infecting bacteria and its pattern of resistance to antibiotics.

Our lab aims to investigate new antimicrobial therapies and strategies to combat bacterial infections with different objectives:. Model of ribonucleotide reductase activity and vitamin B12 availability during P.

Antibacterial activity of two ribonucleotide reductase inhibitors on formed flow cell Pseudomonas aeruginosa biofilm. Vladimir Arion, Department of Inorganic Chemistry.

University of Vienna, Austria. Facultad de Ciencias, Universidad de Granada, Spain. Basas, J. High-dose daptomycin is effective as an antibiotic-lock therapy in a rabbit model of Staphylococcus epidermidis catheterrelated infection.

Antimicrobial Agents and Chemotherapy, 62 2 : e Crespo, A. Aerobic vitamin B12 biosynthesis is essential for pseudomonas aeruginosa class II ribonucleotide reductase activity during planktonic and biofilm growth.

Frontiers in Microbiology, 9 : Article Pressure microinjection system Nikon Inverted fluorescent. Pujol, E. Pentafluorosulfanyl-containing triclocarban analogs with potent antimicrobial activity.

Molecules, 23 11 : Miret-Casals, L. Hydroxylamine derivatives as a new paradigm in the search of antibacterial agents. ACS Omega, 3 12 : Integrative cell and tissue dynamics Xavier Trepat ICREA Research Professor We aim at understanding how physical forces and molecular control modules cooperate to drive biological function.

We develop new technologies to map and perturb the main physical properties that determine how cells and tissues grow, move, invade and remodel.

By combining this physical information with systematic molecular perturbations and computational models we explore the principles that govern the interplay between chemical and physical cues in living tissues.

We study how these principles are regulated in physiology and development, and how they are derailed in cancer and aging. Making cellular forces visible To study cell and tissue dynamics we develop new technologies to measure physical forces at the cell-cell and cell-matrix interface.

By combining these technologies with computational analysis of cell shape and velocity we obtain a full experimental characterization of epithelial dynamics during tissue growth, wound healing and cancer cell invasion.

Tumour invasion by stromal forces Cancer cell invasion and metastasis remain the leading cause of death in patients with cancer.

Both processes are the result of a complex interaction between tumor cells and their microenvironment. One of our main lines of research is to study how tumours exploit the functions non-cancer cells in their microenvironment to invade and metasize.

We focus on the interaction between epithelial cancer cells and Cancer Associated Fibroblasts CAFs , the most abundant cell type in the tumour stroma.

In a recent study we were able to demonstrate that CAFs guide the collective invasion of cancer cells through a physical force.

This force enables CAFs to physically drag cancer cells into the surrounding tissue. Force transmission is mediated by a heterotypic interaction between two different proteins, one located on the surface of cancer cells called E-cadherin, and another expressed on the surface of fibroblasts, called N-cadherin.

Super-stretched cells surrounded by only slightly deformed ones. The cell nucleus is shown in blue, actin filaments in red, and keratin filaments in green.

Optogenetics to control cell mechanics The recent development of optogenetic technologies offers promising possibilities to control signalling pathways with high spatiotemporal resolution.

By expressing genetically encoded light-sensitive proteins, optogenetic technology enables the reversible perturbation of intracellular biochemistry with subcellular resolution.

We have developed optogenetic tools based on controlling the activity of endogenous RhoA to upregulate or downregulate cell contractility. We have shown that these tools enable rapid, local and reversible changes in traction forces, cell—cell forces, and tissue compaction.

We have shown, that changes in cellular forces are paralleled by translocation of the transcriptional regulator YAP, indicating that our tools can be used to control mechanotransductory pathways.

Collective durotaxis: a mechanism for cellular guidance by mechanical cues Directed cell migration is one of the earliest observations in cell biology, dating back to the late XIX century.

Also known as taxis, directed cell migration has been commonly associated with chemotaxis, i. We recently demonstrated a new mode of collective cell guidance by mechanical cues, called collective durotaxis.

This new migration mode emerges only in cell collectives and, strikingly, does not require isolated cells to exhibit gradient sensing.

To study the mechanisms behind this phenomenon, we developed new tools to measure the forces that propel cells during durotaxis at the cell-matrix and cell-cell levels.

Upon combining this new experimental technique with biochemical approaches and theoretical modelling, we concluded that collective durotaxis originates from long-range transmission of contractile intercellular forces.

This mechanism is unique in that the very same machinery that senses the attractant -the actomyosin cytoskeleton- is responsible for propulsion towards it.

As such, collective durotaxis appears to be the simplest and perhaps most primitive mechanism by which a collective system responds to a gradient.

Microfabrication and wound healing. An epithelium of cells of the MDCK line with the marked nuclei: the color ranges from red to green depending on the hase of the cell cycle; red for phase G1, green for phases S, G2 and M.

Shellard, A. Supracellular contraction at the rear of neural crest cell groups drives collective chemotaxis.

Using microfabrication technologies, we designed new ways to decipher the mechanisms of wound healing. By doing so we uncovered a new understanding of how cells move and work together to close a gap in a tissue.

We showed that a new mechanism applies in which cells assemble supracellular contractile arcs that compress the tissue under the wound. Campo, A.

Trepat, X. Mesoscale physical principles of collective cell organization. Nature Physics, 14 7 : Good, M. Cell parts to complex processes, from the bottom up.

Regulation of cell cycle progression by. By combining experiments and computational modeling, we showed that contractions arising from these arcs make the wound heal in a quicker and more robust way.

Stretching epithelial tissues Epithelial tissues are fundamental to life, as they protect the body from radiation, pollutants and pathogens.

We developed a new approach to subject epithelial tissues to very large deformations, up to four times their original size.

As they become progressively deformed, they want to go back to their unstretched state, like a rubber band, or may even break as the tension increases.

We found that epithelial sheets have a different and unusual mechanical behavior. To our surprise, tissues did not break during stretching, and they were able to recover their initial size in a fully reversible way when unstretched.

Understanding this surprising mechanical behavior in epithelial tissues could help us build better artificial organs or new bionic technologies such as organs-on-a-chip.

Breast cancer cells attached to a surface rich in collagen. The actin cytoskeleton can be seen in green, coated with active myosin ppMLC in red, and the cell-cell junctions E-cadherin in blue.

Control of mechanotransduction by molecular clutch dynamics. Trends in Cell Biology, 28 5 : Thottacherry, J.

Labernadie, A. Sticking, steering, squeezing and shearing: cell movements driven by heterotypic. Current Opinion in Cell Biology, 54 Pardo-Pastor, C.

Piezo2 channel regulates RhoA and actin cytoskeleton to promote cell mechanobiological. Dix, C. The role of mitotic cell-substrate adhesion re-modeling in animal cell division.

Developmental Cell, 45 1 : Junior Leader Program. De Pascalis, C. Intermediate filaments control collective migration by restricting traction forces and sustaining cell—cell contacts.

The Journal of Cell Biology, 9 : Kuipers, A. Sehgal, P. Epidermal growth factor receptor and integrins control force-dependent vinculin recruitment to E-cadherin junctions.

Journal of Cell Science, 6 : jcs A hybrid. The Robotic Systems Laboratory is a multidisciplinary environment that supports research in the following areas: Classical conditioning, operant conditioning and learning models based on the Distributed Adaptive Control framework, which has become a standard in the field of artificial intelligence and behavior-based robotics Multi-robot exploration and coordination.

PLoS Computational Biology, 14 11 : e Zamora, R. Arsiwalla, X. Measuring the complexity of consciousness.

Frontiers in Neuroscience, 12 : Article Santos-Pata, D. Human vicarious trial and error is predictive of spatial navigation performance. Frontiers in Behavioral Neuroscience, 12 Article Truschzinski, M.

Emotional and cognitive influences in air traffic controller. Over the past 15 years, SPECS has been developing science-based technology tools to drive perceptual, cognitive, affective and motor systems of the brain to facilitate functional recovery after damage.

Restor Neurol Neurosci and Stroke The Psychophysiology lab studies how humans react to various uni- and multisensory signals — visual, auditory and tactile stimuli.

We assess human responses at different levels using subjective ratings, behavioral data, physiological and brain wave recordings.

Affective chronometry such parameters as the rise time to peak and the recovery time of the emotional waveform Multisensory perception sound, vision, touch Multisensory interactions for emotional stimuli custom sound and video databases are created Sonification of EEG signals Neurofeedback using mixed reality environments processing of eye-gaze in autistic children Hybrid Systems laboratory HLB The HLB is primarily involved in the development, implementation, and analysis of machine-brain-machine interfaces.

Applied Ergonomics, 69

Ines Kussmann - Hautnah: Die Tierklinik – News

Die Besitzer sind in die Tierklinik geeilt, Dr. Vereinbarung Für Notfälle telefonisch erreichbar zwischen und s. Er untersucht die junge Katze und hat zunächst mehrere Vermutungen.

Chemical characterization, antioxidant and antimutagenic evaluations of pigmented corn. Journal of Food Science and Technology , 56 7 , Phytotherapy Research , 33 7 , Understanding the role of active components from plant sources in obesity management.

Journal of the Saudi Society of Agricultural Sciences , 18 2 , The target cells of anthocyanins in metabolic syndrome.

Critical Reviews in Food Science and Nutrition , 59 6 , Cyanidin and cyanidinglucoside derived from Vigna unguiculata act as noncompetitive inhibitors of pancreatic lipase.

Journal of Food Biochemistry , 43 3 , e Jaboticaba: Chemistry and Bioactivity. CyanidinO-glucoside inhibits different enzymes involved in central nervous system pathologies and type-2 diabetes.

South African Journal of Botany , , Effect of purified anthocyanins or anthocyanin-rich extracts on C-reactive protein levels: a systematic review and meta-analysis of randomised clinical trials.

British Journal of Nutrition , 12 , Anthocyanins from Cornus kousa ethanolic extract attenuate obesity in association with anti-angiogenic activities in 3T3-L1 cells by down-regulating adipogeneses and lipogenesis.

Antidiabetic potential of purple okra Abelmoschus esculentus L. Journal of the American College of Nutrition , 37 7 , Anthocyanins isolated from blueberry ameliorates CCl4 induced liver fibrosis by modulation of oxidative stress, inflammation and stellate cell activation in mice.

Food and Chemical Toxicology , , McCarty, Simon B. Iloki Assanga. Ferulic acid may target MyDmediated pro-inflammatory signaling — Implications for the health protection afforded by whole grains, anthocyanins, and coffee.

Medical Hypotheses , , Winter, Erika K. Ross, Heather M. Wilkins, Trisha R. An anthocyanin-enriched extract from strawberries delays disease onset and extends survival in the hSOD1 G93A mouse model of amyotrophic lateral sclerosis.

Nutritional Neuroscience , 21 6 , Journal of Functional Foods , 45 , McDougall, Derek Stewart. Evidence for Health Benefits of Berries.

Total and specific dietary polyphenol intakes and 6-year anthropometric changes in a middle-aged general population cohort. International Journal of Obesity , 42 3 , Anthocyanin Production in Engineered Microorganisms.

Effect of microwaves and ultrasound on bioactive compounds and microbiological quality of blackberry juice.

LWT , 87 , PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation.

Loizzo, Marco Bonesi, Seyed M. Eugenia uniflora fruit red type standardized extract: a potential pharmacological tool to diet-induced metabolic syndrome damage management.

Sigurdson, M. Phenolic Compounds. International Journal of Molecular Sciences , 18 2 , Nutraceuticals and Cognitive Dysfunction.

Azzini, E. Venneria, D. Ciarapica, M. Foddai, F. Intorre, M. Zaccaria, F. Maiani, L. Palomba, L. Barnaba, C. Tubili, G. Maiani, A.

Oxidative Medicine and Cellular Longevity , , Screening of the anthocyanin profile and in vitro pancreatic lipase inhibition by anthocyanin-containing extracts of fruits, vegetables, legumes and cereals.

Journal of the Science of Food and Agriculture , 96 14 , Molecules , 21 10 , Chemistry, Pharmacology and Health Benefits of Anthocyanins. Phytotherapy Research , 30 8 , Antioxidants , 5 2 , Bess, Rachel N.

Carmody, Peter J. The microbial pharmacists within us: a metagenomic view of xenobiotic metabolism. Nature Reviews Microbiology , 14 5 , Vance, Ock K.

British Journal of Nutrition , 8 , Hodge, Mark A. Paulik, Ann Walker, Joyce A. Boucheron, Susan L. McMullen, Dawn S. Gillmor, Derek J. The Role of Anthocyanins in Obesity and Diabetes.

Advances in the study of the health benefits and mechanisms of action of the pulp and seed of the Amazonian palm fruit, Euterpe oleracea Mart.

Hommelberg, Marten H. Tietge, Debby P. Koonen, Peter Heeringa. Mediators of Inflammation , , Bunseki kagaku , 65 6 , Advances in Nutrition , 7 1 , Gardener, Stephanie R.

Rainey-Smith, Ralph N. Journal of Alzheimer's Disease , 50 2 , Whole Grains and Metabolic Syndrome. New acylated anthocyanins from purple yam and their antioxidant activity.

Bioscience, Biotechnology, and Biochemistry , 79 9 , Journal of Functional Foods , 17 , Natural Product Communications , 10 6 , X Kaufman, Steven F.

Tissue bioavailability of anthocyanins from whole tart cherry in healthy rats. Antioxidant properties of fruits of raspberry and blackberry grown in central Europe.

Journal of Bioscience and Bioengineering , 1 , Dual effects of a mixture of grape pomace Campbell Early and Omija fruit ethanol extracts on lipid metabolism and the antioxidant defense system in diet-induced obese mice.

Nutrition Research and Practice , 9 3 , Alvarez-Suarez, Ana M. Quiles, Bruno Mezzetti, Maurizio Battino. Strawberry intake increases blood fluid, erythrocyte and mononuclear cell defenses against oxidative challenge.

Mecocci, C. Tinarelli, R. Schulz, M. Isolation of cyanidin 3-glucoside from blue honeysuckle fruits by high-speed counter-current chromatography.

Composition and inhibitory activities towards digestive enzymes of polyphenolic-rich fractions of Davidson's plum and quandong.

Therapeutic potential of Hibiscus sabdariffa: A review of the scientific evidence. Development and study of a maize cultivar rich in anthocyanins: coloured polenta, a new functional food.

Plant Breeding , 2 , Phytochemical properties and antioxidant capacities of various colored berries. Journal of the Science of Food and Agriculture , 94 2 , Dietary Quercetin and other Polyphenols.

Ribnicky, Diana E. Effects of a high fat meal matrix and protein complexation on the bioaccessibility of blueberry anthocyanins using the TNO gastrointestinal model TIM Food Funct.

Emiliano, Viviane S. Cordeiro, Graziele F. Neto, Cristiane A. Costa, Gilson T. Boaventura, Roberto S. Grape skin extract protects against programmed changes in the adult rat offspring caused by maternal high-fat diet during lactation.

The Journal of Nutritional Biochemistry , 24 12 , Blueberry anthocyanins in health promotion: A metabolic overview.

Journal of Functional Foods , 5 4 , Journal of Food Processing and Preservation , 37 5 , James, Stuart K. Johnson, Linda C. Vegetables containing phytochemicals with potential anti-obesity properties: A review.

Food Research International , 52 1 , Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers.

Food Chemistry , 1 , Macdonald-Clarke, Helen M. Dietary Anthocyanidins and Bone Health. Phytochemical properties and antioxidant capacities of commercial raspberry varieties.

Journal of Functional Foods , 5 1 , Holst, Wendy Russell, Graham Horgan. A single supplement of a standardised bilberry Vaccinium myrtillus L.

Hibiscus sabdariffa L. Trigueros, S. Ugidos, E. Critical Reviews in Food Science and Nutrition , 53 9 , Food Digestion , 3 , Barth, Tatiana C.

Moderate effects of apple juice consumption on obesity-related markers in obese men: impact of diet—gene interaction on body fat content.

European Journal of Nutrition , 51 7 , Food and Chemical Toxicology , 50 9 , Turner, Cassandra M. McDonough, Lloyd W. Effects of Sorghum [Sorghum bicolor L.

Molecules , 17 9 , Short-term supplementation with Aronia melanocarpa extract improves platelet aggregation, clotting, and fibrinolysis in patients with metabolic syndrome.

European Journal of Nutrition , 51 5 , Tiliroside, a glycosidic flavonoid, ameliorates obesity-induced metabolic disorders via activation of adiponectin signaling followed by enhancement of fatty acid oxidation in liver and skeletal muscle in obese—diabetic mice.

The Journal of Nutritional Biochemistry , 23 7 , Tiliroside, a glycosidic flavonoid, inhibits carbohydrate digestion and glucose absorption in the gastrointestinal tract.

Shidler, Krista Dangelo, Sarah C. Villanueva, J. A portable continuous positive airway pressure device that can perform optimally under strenuous conditions.

Torres, M. Aging reduces intermittent hypoxiainduced lung carcinoma growth in a mouse model of sleep apnea. Is telemedicine a key tool for improving continuous positive airway pressure adherence in patients with sleep apnea?

Notari, M. The local microenvironment limits the regenerative potential of the mouse neonatal heart. Science Advances, 4 5 : eaao Alcaraz, J.

Bidirectional mechanobiology between cells and their local extracellular matrix probed by atomic force microscopy.

Seminars in Cell and Developmental Biology, Perea-Gil, I. Head-to-head comparison of two engineered cardiac grafts for myocardial.

Menal, M. Frontiers in Neurology, 9: Article 1 Intermittent hypoxia mimicking sleep apnea increases passive stiffness of myocardial extracellular matrix.

A multiscale study. Frontiers in Physiology, 9: Article Passive stiffness of left ventricular myocardial tissue is reduced by ovariectomy in a post-menopause mouse model.

Bioengineered lungs: A challenge and an opportunity. In the case of animal models we have not only some ethical problems but also the ability to extrapolate data to human conditions is limited and in vitro platforms often do not simulate the complex cell—cell and cell—matrix interactions crucial for regulating cell behaviour.

Biosensors for bioengineering group is focused in a new line of research that has become of extreme importance in the last years.

The idea is to integrate biosensor technology and nanotechnology with stem cell research and with tissue engineering.

Engineered tissues are integrated with biosensing technology to obtain microdevices for detecting cellular responses to external stimuli, monitoring the quality of the microenvironment e.

This research on 3D-functional engineered tissues is expected to develop knowledge of tissue construction and their functions and relation with some human diseases.

These chips could be used in pharmaceutical assays and could be a step toward the ultimate goal of producing in vitro drug testing systems crucial to the medicine and pharmaceutical industry.

Pancreas islet stained in blue for nuclei and red for actin inside a microporous microfibrillated cellulose-gelatin scaffold stained with green.

Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissue. Macromolecular Bioscience, 18 10 : Conference Paper.

We believe that combining several different stimuli in the chip resembles a more realistic environment that nerve cells will encounter in the living animal in normal and disease conditions.

These experiments using Labon-a-Chip models in turn will make future studies on the role of neuronal cells in development and regeneration more accurate and complete.

Thus, we developed a new device able to reproduce the formation of the neuromuscular junction NMJ in lab on chip devices.

In addition, a device designed to analyse axon lesions of cortical neurons was also developed. Current experiments. For example: i on chip lab platform to monitor drug delivery modulating neuronal activity see figure ; ii cortico-spinal chips to develop axon regenerative studies of new drug formulation in collaboration with Imperial College; London ; iii devices to molecular gradient generation for migrating neurons in collaboration with i3A, Zaragoza and, iv in silico 3D modeling for neurodegenerative diseases Alzheimer and Parkinson chip.

Last experiments are focused to reproduce a lab on chip devices to analyse and to reproduce ALS models in collaboration with Biodonostia Hospital Figure 2.

However, the basis of the spreading process remains poorly understood although cell to cell transport via. Experimental evidence suggests that providing a permissive extracellular environment containing cellular or biomaterial bridges is not enough to increase axon regrow to obtain functional regeneration, supporting the importance of clarifying the complex transcriptional response below these signalling pathways.

As an alternative, activating or recapitulating the developmental program of the lesioned neuron to. Hervera, A. Nature Cell Biology, 20 3 : Role of cellular prion protein in interneuronal amyloid transmission.

Progress in Neurobiology, Urrea, L. Molecular Neurobiology, 55 3 : Optogenetics is currently the state-of-the-art method for activity-based nervous system research, allowing more specific cellular stimulations by light in a less invasive techniques compared to classical electrical stimulation, but leading to a more tailored physiological responses.

In our laboratory, we started to analyse whether optical stimulation of lesioned neurons is able to enhance axonal growth or sprouting. These experiments were performed on lesioned cortical neurons and in vivo.

Matamoros-Angles, A. Molecular Neurobiology, 55 4 : Llorens, F. Vivancos, A. Regional and subtype-dependent miRNA signatures in sporadic Creutzfeldt-Jakob disease are accompanied by alterations in miRNA silencing machinery and biogenesis.

PLoS Pathogens, 14 1 : e Garcia-Esparcia, P. Brain Pathology, 28 1 : Ferrer, I. Brain Pathology, 28 6 : — Elisabeth Engel page 13 , Prof.

Josep Samitier, page 61 , Prof. Xavier Trepat, page 75 and Prof. Antonella Consiglio and Dr. Beth L. Mata, A. New functions of Semaphorin 3E and its receptor PlexinD1 during developing and adult hippocampal formation.

Franco, R. N-methyl-D-aspartate receptor link to the MAP kinase pathway. Frontiers in Molecular Neuroscience, 11 : Article Tomas-Roig, J.

Effects of repeated. Badiola-Mateos, M. Challenges and future prospects on 3D in-vitro modeling of the neuromuscular circuit.

Frontiers in Bioengineering and Biotechnology, 6 Article Cellular and molecular mechanobiology Pere Roca-Cusachs Every time we blink, move a hand, draw a breath, or walk, cells in our body exert, transmit, withstand, and detect forces.

This mechanical interaction with the environment determines how cells proliferate, differentiate, and move, and regulates development, tumorigenesis or wound healing.

Just like biochemical stimuli initiate signaling cascades, mechanical forces affect the links and conformation of a network of molecules connecting cells to the extracellular matrix.

Our research aims precisely at unraveling the mechanisms that these molecules use to detect and respond to mechanical stimuli like forces or tissue rigidity, triggering downstream cell responses.

To this end, we combine biophysical techniques like magnetic tweezers, Atomic Force Microscopy, traction microscopy, and microfabricated force sensors with molecular biology, advanced optical microscopy, and theoretical modelling.

Sensing rigidity: Using this multi-disciplinary approach, we have recently unveiled a molecular mechanism that cells employ to detect and respond to the rigidity of their environment, which could be crucial in breast tissue.

Cell Biol. This affects force transmission from the environment to cells, and also within different cell components.

Recently, we have begun to explore how force transmission to the nucleus affects the dynamics of transcriptional regulators, such as YAP Elosegui-Artola et al.

Sensing the environment: We are currently expanding on the idea of the molecular clutch, to explore how cell molecular engines sense not only mechanical rigidity, but other important parameters from their environment: for instance, the composition and distribution of ligands.

Artistic rendering of a cell attaching to a substrate coated with a gold nano-pattern array, used to study how cells detect spatial cues From Oria et al.

In this regard, we recently uncovered that this concept can explain how cells sense the spatial distribution of ligands in the extracellular matrix Oria et al.

We have also demonstrated that cell-cell force transmission, mediated by a molecular clutch, is essential for cells to sense gradients in stiffness Sunyer et al.

Ultimately, when we determine the molecular mechanisms that communicate cells with their environment, we will understand how forces determine development when things go right, and tumor formation when they go wrong.

The membrane as a mechanosensor: Due to its mechanical properties, the plasma membrane itself can respond to forces and act as a mechanosensor.

Recently, we have shown that cell membranes can use purely physical principles to adapt their shape in response to mechanical forces Kosmalska et al.

We are currently studying how cells harness this physical membrane behavior to respond to signals from their environment. Cartoon depicting how force transmission to the nucleus affects nuclear pores, leading to nuclear protein import from Elosegui-Artola et al.

Elosegui-Artola, A. Control of. Trends in Cell Biology, 28 5 : Thottacherry, J. Mechanochemical feedback and control of endocytosis and membrane tension.

Nature Communications, 9 Gauthier, N. Mechanosensing at integrin-mediated cell—matrix adhesions: from molecular to. Current Opinion in Cell Biology, 50 Bennett, M.

Molecular clutch drives cell response to surface viscosity. Roca-Cusachs, P. Cell scientist to watch - Pere Roca-Cusachs. Journal of Cell Science, 16 : jcs Escribano, J.

A hybrid computational model for collective cell durotaxis. Biomechanics and Modeling in Mechanobiology, 17 4 : Nanobioengineering Josep Samitier The Nanobioengineering group is a truly multidisciplinary team composed by researchers coming from very diverse backgrounds working together in applying nanotechnology for the development of new biomedical systems and devices, mainly for diagnostic purposes, and integrated microfluidic Organ-on-aChip devices for the study of organ physiology, disease etiology, or drug screening.

The main research activities of the group include the engineering and biochemical functionalization of biomaterials integrated with microfluidics systems.

The bioengineered microdevices are used to study cell responses to biomolecular compounds applied to Organon-Chip devices, or for the development of new Lab-on-aChip based biosensors.

The goal is to fabricate microsystems containing living cells that recapitulate tissue and organ level functions in vitro and new portable diagnosis devices that can be used as Point-of-Care systems.

The projects carried out by the group are focused on clinical and industrial problems and are related to three convergent research lines:.

Tumor Cancer on a chip in vitro development Microfluidic Vessel on-a-chip for screening drug delivery systems. Katuri, J. Directed flow of micromotors through alignment interactions with micropatterned ratchets.

ACS Nano, 12 7 : Gorostiza, P. Molecular architecture for DNA wiring. Biosensors and Bioelectronics, Blood-based cancer biomarkers in liquid biopsy: A.

U nanoET-leukemia Nanoconductance of electron transfer proteins of the respiratory chain. International Journal of Molecular Sciences, 19 10 : Silva, N.

Nanomaterials, 8 12 : Cross-fertilization of Key Enabling Technologies: An empirical study of nanotechnology-related projects.

Dana-Farber Cancer Institute, Inc. Journal of Engineering and Technology Management, 49 Multi-disciplinarity breeds diversity: the influence of innovation project characteristics on diversity creation in nanotechnology.

Journal of Technology Transfer, 43 2 : Juan C. Producing 3D biomimetic nanomaterials for musculoskeletal system regeneration.

Sampietro, Politecnico di Milano, Italy Prof. Molly M. Miguel A. Industry partners: Biokit S. Werfen group ; Genomica S. Zeltia group ; Tallers Fiestas S.

We are interested in fundamental studies of active matter, the use of nanobots for future nanomedicine and environmental applications and the bioengineering of new devices based on hybrid systems.

Nano-Bio Team The use of enzyme catalysis is emerging as an attractive alternative to power micro- and nanomachines due to their unique features including biocompatibility, versatility and fuel bioavailability.

Our group has demonstrated the use of different enzymes, including urease and glucose oxidase, to generate active propulsion of nano- and microparticles, paving the way towards new applications of artificial active matter in biomedicine.

In particular, we take advantage of the 3D bioprinting technique to develop bio-robotic systems composed of skeletal muscle cells embedded. Smart micro- and nanorobots are able to swim, monitor their own activity, sense their environment and deliver drugs to 3D bladder cancer spheroids using biocompatible and bioavailable fuels such as urea.

These materials can act as scaffolds, support, or flexible parts, as well as be responsive upon certain stimuli.

By controlling the contractions of skeletal muscle cells via electric fields, we can measure the forces exerted by these bio-actuators against artificial 3D-printed posts.

Using this setup, we have performed studies on the adaptability of bio-actuators after applying different training protocols and we have observed how their force generation and gene expression can adapt to the frequency of stimulation and stiffness of the artificial posts.

Active matter in complex systems We study colloidal suspensions of Pt-coated silica particles as a model system of synthetic active matter.

These systems have mostly been studied in homogeneous environments until now. Our interest lies in observing these systems in more complex settings, such as near interfaces, complex media or with flow involved.

Since the self-propelled particles generate chemical and hydrodynamic fields around them, they interact in complex ways with flows and nearby surfaces that often leads to.

Upon electrical stimulation, the muscle can contract, bend the post and their force can be calculated. Fundamental aspects of enzyme-powered micro- and nanoswimmers.

Accounts of Chemical Research, 51 11 : — Influence of enzyme quantity and distribution on the self-propulsion of non-Janus urease-powered micromotors.

Journal of the American Chemical Society, 30 : Vilela, D. Medical imaging for the tracking of micromotors.

When flow is present, particles also behave different as they reorient perpendicular to the flow.

Environmental applications of micro-nano motors Artificial micromotors, based on bubble self-propulsion have demonstrated to be able to mix solutions and.

These micromotors are mostly based on two main structures, tubular and spherical. On the other hand, electrodeposited microjets, which are smaller.

Catalytically active colloids generate chemical gradients and interact with their nearby surfaces while they swim.

When they swim in capillaries where a flow is imposed, they present a cross stream migration deviating from straight trajectories, perpendicular to the flow lines.

This type of motion resembles, in some aspects, the motion of biological microswimmers. ACS Nano, 12 2 : Wang, X.

Fuel-free nanocap-like motors actuated under. Advanced Functional Materials, 28 25 : Enzyme-powered nanobots enhance anticancer drug delivery.

Advanced Functional Materials, 28 Xuan, M. Noncontinuous super-diffusive dynamics of a light-activated nanobottle motor.

Angewandte Chemie International Edition, 57 23 : Cross-stream migration of active particles. Science Advances, 4 1 : eaao In this case, the metal is adsorbed and removed from the contaminated water.

The metal can thereafter be desorbed and the microjets used again. In order to target other water pollution problems, such as microorganism contamination, we have developed spherical microbots that can kill bacteria while they swim.

These microbots have a Janus structure based on spherical magnesium microparticles, able to dissolve in water producing hydrogen bubbles, covered in one of their faces by Fe, Au and AgNPs which provide magnetic, bacteria attachment and bactericidal properties to the microjets.

Towards scaling-up of the micromotor synthesis for cleaning large volumes of water, we have fabricated micromotors using exclusively chemical methods such as, precipitation, reduction and sol-gel chemistry.

These micromotors are based on a silica microtubular structure which contains an inner-layer of a catalytic material PtNPs or MnO2 capable of removing pollutants efficiently from water while they swim in the presence of hydrogen peroxide.

The external decoration of these structures with magnetic nanoparticles provides for good magnetic control. Finally, magnetic and catalytic micromotors formed by the aggregation of cobalt ferrite nanoparticles were synthesized to remove antibiotics from water.

All these micromotors, due to their magnetic properties can be removed from the solution after finishing their targeting action by the application of an external magnetic field.

Villa, K. Metal-oxide-based microjets for the simultaneous removal of organic pollutants and heavy metals. Romeo, A. Inkjet printed flexible non-enzymatic glucose sensor for tear fluid analysis.

Applied Materials Today, 10 Core-shell microspheres for the ultrafast degradation of estrogen hormone at neutral pH. RSC Advances, 8 11 : Conference Papers Mestre, R.

Paris, France Point-of-care diagnostics allows decentralizing clinical diagnostic practices and monitoring health out of specialized hospital settings.

Advantages of such decentralization are improved quality of life of patients, enhanced therapeutic efficacy thanks to more frequent tests, and lower overall cost of the health system.

We develop flexible biochemical sensors for non-invasive and cost-effective monitoring of analytes in biological fluids alternative to blood, e.

We combine electrochemical electrochemical sensors with microfluidics and electronics to achieve fully integrated devices, that are well suited for low-cost, portable and user-friendly medical diagnostics.

Dietrich, Dr. Popescu, M. Tasinkevych, Dr. Analytes are quickly quantified by electrochemical techniques. Gabriel and Prof. Bacterial Infections: Antimicrobial Therapies Eduard Torrents Infectious diseases constitute a tenacious and major public health problem all over the world.

The emergence and increasing prevalence of bacterial strains that are resistant to available antibiotics demand the discovery of new therapeutic approaches.

There is an urgent need for reliable and rapid detection of infecting bacteria and its pattern of resistance to antibiotics.

Our lab aims to investigate new antimicrobial therapies and strategies to combat bacterial infections with different objectives:. Model of ribonucleotide reductase activity and vitamin B12 availability during P.

Antibacterial activity of two ribonucleotide reductase inhibitors on formed flow cell Pseudomonas aeruginosa biofilm. Vladimir Arion, Department of Inorganic Chemistry.

University of Vienna, Austria. Facultad de Ciencias, Universidad de Granada, Spain. Basas, J. High-dose daptomycin is effective as an antibiotic-lock therapy in a rabbit model of Staphylococcus epidermidis catheterrelated infection.

Antimicrobial Agents and Chemotherapy, 62 2 : e Crespo, A. Aerobic vitamin B12 biosynthesis is essential for pseudomonas aeruginosa class II ribonucleotide reductase activity during planktonic and biofilm growth.

Frontiers in Microbiology, 9 : Article Pressure microinjection system Nikon Inverted fluorescent. Pujol, E.

Pentafluorosulfanyl-containing triclocarban analogs with potent antimicrobial activity. Molecules, 23 11 : Miret-Casals, L. Hydroxylamine derivatives as a new paradigm in the search of antibacterial agents.

ACS Omega, 3 12 : Integrative cell and tissue dynamics Xavier Trepat ICREA Research Professor We aim at understanding how physical forces and molecular control modules cooperate to drive biological function.

We develop new technologies to map and perturb the main physical properties that determine how cells and tissues grow, move, invade and remodel.

By combining this physical information with systematic molecular perturbations and computational models we explore the principles that govern the interplay between chemical and physical cues in living tissues.

We study how these principles are regulated in physiology and development, and how they are derailed in cancer and aging.

Making cellular forces visible To study cell and tissue dynamics we develop new technologies to measure physical forces at the cell-cell and cell-matrix interface.

By combining these technologies with computational analysis of cell shape and velocity we obtain a full experimental characterization of epithelial dynamics during tissue growth, wound healing and cancer cell invasion.

Tumour invasion by stromal forces Cancer cell invasion and metastasis remain the leading cause of death in patients with cancer. Both processes are the result of a complex interaction between tumor cells and their microenvironment.

One of our main lines of research is to study how tumours exploit the functions non-cancer cells in their microenvironment to invade and metasize.

We focus on the interaction between epithelial cancer cells and Cancer Associated Fibroblasts CAFs , the most abundant cell type in the tumour stroma.

In a recent study we were able to demonstrate that CAFs guide the collective invasion of cancer cells through a physical force. This force enables CAFs to physically drag cancer cells into the surrounding tissue.

Force transmission is mediated by a heterotypic interaction between two different proteins, one located on the surface of cancer cells called E-cadherin, and another expressed on the surface of fibroblasts, called N-cadherin.

Super-stretched cells surrounded by only slightly deformed ones. The cell nucleus is shown in blue, actin filaments in red, and keratin filaments in green.

Optogenetics to control cell mechanics The recent development of optogenetic technologies offers promising possibilities to control signalling pathways with high spatiotemporal resolution.

By expressing genetically encoded light-sensitive proteins, optogenetic technology enables the reversible perturbation of intracellular biochemistry with subcellular resolution.

We have developed optogenetic tools based on controlling the activity of endogenous RhoA to upregulate or downregulate cell contractility.

We have shown that these tools enable rapid, local and reversible changes in traction forces, cell—cell forces, and tissue compaction.

We have shown, that changes in cellular forces are paralleled by translocation of the transcriptional regulator YAP, indicating that our tools can be used to control mechanotransductory pathways.

Collective durotaxis: a mechanism for cellular guidance by mechanical cues Directed cell migration is one of the earliest observations in cell biology, dating back to the late XIX century.

Also known as taxis, directed cell migration has been commonly associated with chemotaxis, i. We recently demonstrated a new mode of collective cell guidance by mechanical cues, called collective durotaxis.

This new migration mode emerges only in cell collectives and, strikingly, does not require isolated cells to exhibit gradient sensing. To study the mechanisms behind this phenomenon, we developed new tools to measure the forces that propel cells during durotaxis at the cell-matrix and cell-cell levels.

Upon combining this new experimental technique with biochemical approaches and theoretical modelling, we concluded that collective durotaxis originates from long-range transmission of contractile intercellular forces.

This mechanism is unique in that the very same machinery that senses the attractant -the actomyosin cytoskeleton- is responsible for propulsion towards it.

As such, collective durotaxis appears to be the simplest and perhaps most primitive mechanism by which a collective system responds to a gradient.

Microfabrication and wound healing. An epithelium of cells of the MDCK line with the marked nuclei: the color ranges from red to green depending on the hase of the cell cycle; red for phase G1, green for phases S, G2 and M.

Shellard, A. Supracellular contraction at the rear of neural crest cell groups drives collective chemotaxis. Using microfabrication technologies, we designed new ways to decipher the mechanisms of wound healing.

By doing so we uncovered a new understanding of how cells move and work together to close a gap in a tissue. We showed that a new mechanism applies in which cells assemble supracellular contractile arcs that compress the tissue under the wound.

Campo, A. Trepat, X. Mesoscale physical principles of collective cell organization. Nature Physics, 14 7 : Good, M. Cell parts to complex processes, from the bottom up.

Regulation of cell cycle progression by. By combining experiments and computational modeling, we showed that contractions arising from these arcs make the wound heal in a quicker and more robust way.

Stretching epithelial tissues Epithelial tissues are fundamental to life, as they protect the body from radiation, pollutants and pathogens.

We developed a new approach to subject epithelial tissues to very large deformations, up to four times their original size.

As they become progressively deformed, they want to go back to their unstretched state, like a rubber band, or may even break as the tension increases.

We found that epithelial sheets have a different and unusual mechanical behavior. To our surprise, tissues did not break during stretching, and they were able to recover their initial size in a fully reversible way when unstretched.

Understanding this surprising mechanical behavior in epithelial tissues could help us build better artificial organs or new bionic technologies such as organs-on-a-chip.

Breast cancer cells attached to a surface rich in collagen. The actin cytoskeleton can be seen in green, coated with active myosin ppMLC in red, and the cell-cell junctions E-cadherin in blue.

Control of mechanotransduction by molecular clutch dynamics. Trends in Cell Biology, 28 5 : Thottacherry, J. Labernadie, A. Sticking, steering, squeezing and shearing: cell movements driven by heterotypic.

Current Opinion in Cell Biology, 54 Pardo-Pastor, C. Piezo2 channel regulates RhoA and actin cytoskeleton to promote cell mechanobiological.

Dix, C. The role of mitotic cell-substrate adhesion re-modeling in animal cell division. Developmental Cell, 45 1 : Junior Leader Program.

De Pascalis, C. Intermediate filaments control collective migration by restricting traction forces and sustaining cell—cell contacts. The Journal of Cell Biology, 9 : Kuipers, A.

Sehgal, P. Epidermal growth factor receptor and integrins control force-dependent vinculin recruitment to E-cadherin junctions.

Journal of Cell Science, 6 : jcs A hybrid. The Robotic Systems Laboratory is a multidisciplinary environment that supports research in the following areas: Classical conditioning, operant conditioning and learning models based on the Distributed Adaptive Control framework, which has become a standard in the field of artificial intelligence and behavior-based robotics Multi-robot exploration and coordination.

PLoS Computational Biology, 14 11 : e Zamora, R. Arsiwalla, X. Measuring the complexity of consciousness.

Frontiers in Neuroscience, 12 : Article Santos-Pata, D. Human vicarious trial and error is predictive of spatial navigation performance.

Frontiers in Behavioral Neuroscience, 12 Article Truschzinski, M. Emotional and cognitive influences in air traffic controller.

Over the past 15 years, SPECS has been developing science-based technology tools to drive perceptual, cognitive, affective and motor systems of the brain to facilitate functional recovery after damage.

Restor Neurol Neurosci and Stroke The Psychophysiology lab studies how humans react to various uni- and multisensory signals — visual, auditory and tactile stimuli.

We assess human responses at different levels using subjective ratings, behavioral data, physiological and brain wave recordings.

Affective chronometry such parameters as the rise time to peak and the recovery time of the emotional waveform Multisensory perception sound, vision, touch Multisensory interactions for emotional stimuli custom sound and video databases are created Sonification of EEG signals Neurofeedback using mixed reality environments processing of eye-gaze in autistic children Hybrid Systems laboratory HLB The HLB is primarily involved in the development, implementation, and analysis of machine-brain-machine interfaces.

Applied Ergonomics, 69 Pacheco, D. Long-term spatial clustering in free recall. Memory, 26 6 : — Hints vs distractions in intelligent tutoring systems: Looking for the proper type of help.

Freire, I. Fibla, M. Modeling the formation of social conventions in multi-agent populations. Fischer, T. Frontiers in Robotics and AI, 5 22 : Article 22 Moulin-Frier, C.

DAC-h3: A proactive robot cognitive architecture to acquire and express knowledge about the world and the self. Conference paper Arsiwalla, X.

Are brains computers, emulators or simulators? The interdisciplinary nature of the study of hybrid systems lies at the intersection of different research areas, namely: Computational neuroscience Electronics.

The SPECS research group has been pioneering this approach over the last 15 years and grounded it in its fundamental research in psychology and neuroscience.

In collaboration with the Bergen-Belsen memorial site and Prof. Habbo Knoch, this paradigm has been elaborated to conserve and present the history of the Bergen Belsen concentration camp.

Technology evolves and advances faster than ever in all aspects of our society. Thus, it is important that the next generations of students learn as much as possible about emerging technology and stay competitive.

Existing memorial sites or museums offer a traditional historiographical approach. We propose to use virtual and augmented reality techniques to reconstruct sites of World War-II crimes and their interrelated structures.

Challenges of machine learning for living machines. SPECS contributes to the education of the next generations by combining platforms for training and outreach activities, facilitating multidisciplinary education and innovation by sharing the value of convergent science, excellence, and societal impact.

We have developed Educational Robotics programs for students of the primary and secondary school, as well as courses to train teachers and young adults.

Interaction Technology There is a growing interest in understanding creativity from a more neuroscientific point of view, so to say, to disclose the neural basis of creativity we will need great insights on how the brain elaborates the process of human thought.

Computer Science. Rhodes, Greece Insect behavioral evidence of spatial memories during environmental reconfiguration. Maier, M.

Depression modulates attentional processing after stroke. A temporal estimate of integrated information for intracranial functional connectivity.

Embodied artificial intelligence through distributed adaptive control: An integrated. Narrative in interactive mixed reality environment Multimedia installations: affect-based self-generated media content Interaction Technology There is a growing interest in understanding creativity from a more neuroscientific point of view, so to say, to disclose the neural basis of creativity we will need great insights on how the brain elaborates the process of human thought.

Narrative in interactive mixed reality environment Multimedia installations: affect-based self-generated media content Mixed-reality lab The Mixed-reality lab serves a threefold research agenda: Understand human behavior in a mixed-reality context framework.

Lisbon, Portugal Book Prescott, T. Living machines: A handbook of research in biomimetics and biohybrid systems.

Oxford, UK. Oxford Scholarship Vouloutsi, V. Biomimetic and Biohybrid Systems. Lausanne, Switzerland. Springer International Publishing Build mixed-reality applications based on neurobiological understanding and methodologies such as the BrainX3 Test neurobiological models by deploying them in control of mixed-reality systems.

Book chapter Arsiwalla, X. What is the physics of intelligence? In: Frontiers in Artificial Intelligence and Applications ed.

Falomir, Z. Volume Artificial Intelligence Research and Development: Limits of multi-agent predictive models in the formation of social conventions.

Pfeifer, R. The challenge of autonomous agents: Pitfalls and how to avoid them. Steels, L. In: Biomimetic and Biohybrid Systems ed.

Lausanne, Switzerland, Springer International Publishing. Herreros, I. Learning and control. Living machines: A handbook of research in biomimetics and biohybrid systems ed.

Prescott, T. Oxford, UK, Oxford Scholarship: Lepora, N. A roadmap for Living Machines research. In: Living machines: A handbook of research in biomimetics and biohybrid systems ed.

Living machines: An introduction. Robotics lab Codi-Bot, the musical robot that teaches you how to program Collective machine cognition: Autonomous dynamic mapping and planning using a hybrid team of aerial and ground-based robots Robots: Humanoids Pepper, iCub and 3D printed robots, Drones Rehabilitation Gaming Systems BrainX3 Digital applications for Cultural heritage.

Verschure, P. The architecture of mind and brain. A chronology of Distributed Adaptive Control. A living machines approach to the sciences of mind and brain.

Emotions and self-regulation. Open Innovation Lab Direct towards the patient and the market: The Open Innovation Lab for Bioengineering is an IBEC initiative promoting market-oriented research activities by providing co-working lab and office space to help high-potential initiatives funded by external investment capital to go further and faster to the market.

The initiative is open to companies and investors interested in setting up a new research and development unit with a clear market objective.

The goal is to build an ecosystem in bioengineering that empowers entrepreneurial scientists to grow quickly, while maximizing capital efficiency.

The unit will develop a new system for assisted reproduction programmes to predict the success rate of embryos for implantation. Selecting embryos is a critical aspect of this treatment that at the moment is left largely to chance, leading to disappointment and suffering for many patients.

Our goal is to understand the mechanisms controlling mammalian embryo implantation and use that information to provide solutions that improve in vitro fertilization IVF.

The embryonic development of humans and mammals in general requires the implantation of the embryo into the walls of the mother uterus. Implantation involves the attachment of the embryo to the uterine lining, termed endometrium, and the invasion of the tissue to form the placenta.

However, despite the central role of implantation in human fertility, the process is still elusive to experimentation because of its inaccessibility.

In our lab we use bioengineering methods to create 3D environments that support embryonic development outside of the mother uterus.

Our systems are accessible to imaging tools which allow us to interrogate the genetics, metabolomics and mechanics of the embryo in a high.

Using our systems we are capable to i improve embryo culture conditions and ii diagnose embryos with improved implantation potential. Due to the high translational component of our research, we have established collaboration contracts with the pharma industry, hospitals and venture capital to bring our technology to the clinics and the market.

Our Open Lab is a multidisciplinary environment where biologists, biophysicists, clinicians and business developers synergize to create a unique environment shaped by science and entrepreneurship.

Open Lab: Bioengineering in Reproductive Health Our lab studies the development of human and mouse embryos. Our systems are accessible to imaging tools which allow us to interrogate the genetics, metabolomics and mechanics of the embryo in a high throughput manner.

Due to the high translational component of our research, we have. Enhanced Number and Brightness a novel Imaging technique reveals protein dynamics in live cells Proteins continuously interact with other proteins in the cell yet revealing these interactions in live-cell microscopy is technically challenging.

Using statistical tools we designed the Enhanced Number and Brightness method , which quantifies and maps protein aggregation in live cells from high resolution movies.

Several fluorescence microscopy methods can be used to measure protein interactions in cells. However, most of the methods struggle to capture both the diversity of these interactions across the entire cellular area and their dynamics over time.

To overcome these limitations,. The Enhanced Number and Brightness method implements two algorithms, the first one uses statistical tools to extract the distribution of protein oligomers monomers, dimers, trimers and so on in single pixels, thus revealing protein diversity across an entire cell image.

The second one corrects the fluorescence intensity loss due to continuous light exposure, which enables measurements over prolonged periods of time.

By combining these two algorithms, Enhanced Number and Brightness allows extended live imaging, providing dynamic maps of protein oligomerization overlaid on top of the corresponding cell image.

Enhanced Number and Brightness provides oligomerization maps color-coded according to reference bar during time-lapse movies. The image shows from left to right the dynamics of Eph receptor aggregation after stimulation with the ligand Ephrin.

Each row depicts a cell stimulated with different types of ligands i. They are based in the university premises and working on topics that are of interest, or complementary, to IBEC research areas.

They also have the option to submit project proposals and scientific papers with IBEC affiliation. Associated Researchers are approved by the International Scientific Committee, which also evaluates their performance on a regular basis.

He has been awarded the O. He has also been the Timoshenko Visiting Scholar at Stanford. His research goal is to develop theories and computational methods to understand the small-scale mechanics of materials and biological systems, with a recent emphasis on cell and tissue mechanobiology and bio-inspired materials.

Her research interests include the design and development of new biomaterials for bone regeneration, bone tissue engineering and drug delivery. She is involved also in new biofabrication strategies, including injectable scaffolds for bone tissue engineering, bioinspired substrates and 3D printing of regenerative medical implants.

She has been involved in numerous national and European research projects and participated in the organisation of scientific events in the area of biomaterials and bioceramics.

She is author of more than articles in peer-reviewed International journals as well as of 9 patents. In she founded the spin-off company Mimetis Biomaterials.

The group also embarked on a technology transfer venture together with two biopharmaceutical companies, CZV Veterinaria and MEVET, to obtain strains of Salmonella with weakened virulence, which can then be used to develop a vaccine to reduce the incidence of the infection in poultry farms.

His currents research projects are: i the analysis of enterobacterial genomes to better understand bacterial virulence and to identify new targets for novel vaccines, and ii the development of a novel approach to combat plasmid-mediated antibiotic resistance.

Miquel, J. Retaining or excising the supraspinatus tendon in complex proximal humeral fractures treated with reverse prosthesis: a biomechanical analysis in two different designs.

Archives of Orthopaedic and Trauma Surgery, 11 : Sadowska, J. Effect of nano-structural properties of biomimetic hydroxyapatite on osteoimmunomodulation.

Biomaterials, Sadowska, J. In vitro response of mesenchymal stem cells to. Acta Biomaterialia, 76 Raymond, S. Accelerated hardening of nanotextured 3D-plotted self-setting calcium phosphate inks.

Acta Biomaterialia, 75 He pursues two parallel research tracks. On the one hand, he develops innovative nonlinear signal analysis techniques. These techniques aim, for example, at the detection of non-random structure in complex dynamics or the characterization of interactions in networks of dynamics.

On the other hand, he applies these techniques to real-world biomedical signals. Here an emphasis is placed on different types of electrophysiological recordings from epilepsy patients.

During his career he was affiliated with the Neurophysics group of K. Lehnertz and C. Since , he is a tenured associate professor at this Department.

Ralph G. Andrzejak has published over 60 publications in leading journals of physics, neuroscience, neurology, and engineering.

In Google Scholar the publications of Prof. Andrzejak reach more than citations h-index: He is principal investigator of several prestigious research projects receiving funding from Spanish, German and European Institutions.

Barba, A. Osteogenesis by foamed and 3D-printed nanostructured calcium phosphate scaffolds: Effect of pore architecture. Acta Biomaterialia, 79 All-inone trifunctional strategy: A cell adhesive, bacteriostatic and bactericidal coating for titanium implants.

Diez-Escudero, A. Heparinization of beta tricalcium phosphate: Osteo-immunomodulatory effects. Advanced Healthcare Materials, 7 5 : Khurana, K.

Plasma polymerized bioceramics for drug delivery: Do surface changes alter biological behaviour? European Polymer Journal, Guillem-Marti, J.

Nanomedicine, 13 8 : Qi, Y. Effects of molecular weight and concentration of poly acrylic acid on biomimetic mineralization of collagen.

Prieto, A. Evolution of bacterial global modulators: Role of a novel H-NS paralogue in the enteroaggregative escherichia coli strain Stringent response and AggR-dependent virulence regulation in the enteroaggregative.

Frontiers in Microbiology, 9 : Article Hüttener, M. Tetracycline alters gene expression in Salmonella strains that harbor the Tn10 transposon.

Environmental Microbiology Reports, 10 2 : Ralph G. Andrzejak Andrzejak, R. Mean field phase synchronization between chimera states.

Active superelasticity in three-dimensional epithelia of controlled shape. Core Facilities Isabel Oliveira IBEC provides its scientists with extensive research facilities and a scientific—technical infrastructure distributed over interdisciplinary open lab spaces.

It is designed and managed to facilitate investigation and promote the cooperation and exchange of knowledge between IBEC researchers from different fields of expertise.

Furthermore, with the purpose of installing new scientific equipment, refurbishment of several spaces has been carried out during the year.

The Institute leaves MAY — Prof. This new space is occupied by the Finances Unit. JULY — Prof. The laboratory was designed, considering the research activity requirements of the group.

Several spaces dedicated to specific activities, for Instance, chemistry, cell cultural and confocal microscopy were configurated. Benedetta Bolognesi, new junior group leader, opened her Samuel Ojosnegros.

Manage the general maintenance and organization of To keep the overall standard organization of the labs and facilitate the research, the following services are included:.

Inform and train the new incomers about the organization and safety inside IBEC labs, guiding them through the different spaces. Management, preventive and corrective maintenance of shared areas inside the labs and common equipment distributed all over IBEC laboratories.

Reception and documentation purchase related paperwork and inventory databases of the equipment bought by the research groups. The objective is, on the one hand, to obtain laboratory consumables at low cost, due to high amount orders, and on the other hand, to optimize the in lab storage space for this type of consumables, as there is no stock accumulated in the laboratories.

Lab coats laundry management. Organization and preparation of laboratories waste for removal. Advice on doubts that may arise regarding the classification of the residues.

Small equipment available: Scales, heating baths, thermoblocks, thermomixers, centrifuges, magnetic stirrers with or without heater, platform shakers, ultrasonic baths, pHmeters, conductimeter, incubators, ovens, water purification systems, UV tip cleaner, sterilize miniclave, puller machine, etc.

It is equipped with several Class-II biosafety cabinets and Co2 incubators, with one incubator prepared for complex experiments with cells that include extra equipment, and routine equipment for cell culture.

Due to the demand of "alternative" Co2 incubators, suitable to carry out experiments with cells and devices with complex configurations, in September , another large incubator was added to the space and dedicated to this type of experiments.

Also, researchers have access to an inverted microscope and a stereomicroscope, both with epifluorescence option and camera for image caption.

Zgoda, Mikhail V. Unter : Allgemein. We have proposed methods to estimate the limit Cinnemax detection in Kino Weimar Programm chemical sensors using univariate Hofnarr Englisch multivariate calibration models 7. Chibichian, S. We have demonstrated for the first time two-photon stimulation of neurons and astrocytes with azobenzene-based photoswitches. Over the past 15 years, Scouts Vs. Zombies Kinox has been developing science-based technology tools to drive perceptual, cognitive, affective and motor systems of the brain to facilitate functional recovery after damage. Analytica Chimica Acta, Antiobesity and lipid lowering effects of Glycyrrhiza The Division Beste Waffe Experimental and computational studies. Patterson, Jorge D. What is the physics of intelligence? We found that epithelial sheets have a different and unusual mechanical behavior. Proteomic evaluation of myofibrillar carbonylation in chilled fish mince and Bs.To/Serie/The-Walking-Dead/6 inhibition by catechin. Lab technician Angel Blanco Active matter in complex systems We study colloidal suspensions Brühl Cinema Zeitz Pt-coated silica particles as a model system of synthetic active matter. Analytical and Bioanalytical Chemistry8 BCN Peptides, S. Matamoros-Angles, A. Scheinbar hat er sich bei seinem Ausflug schwer verletzt. In der Tierklinik übernimmt Tierärztin Sandra Bridget Jones Baby 28 38 18 Fax: 28 38 17 E-Mail: dr. Tierarzt Dr. Vereinbarung "Kleintierpraxis Sinsdorf". You can set your browser to block these cookies, but some parts of the website may not work. Bislang ohne Ergebnis. Mo: Di: Mi: Do: Fr: Sa: nach Vereinbarung. ET Safwat Ali Hassan. Heute geht es um die folgenden Fälle: Golden Retriever 'Yosie': Golden Retriever Dame "Yosie" hat sich einen Kreuzbandriss zugezogen und muss dringend in der Tierklinik untersucht werden. Folge 52 noch keine Wertung. In der Tierklinik holen sie sich Rat bei Dr. Bevor Sie fortfahren Vereinbarung "Kleintierpraxis Ehestorfer Weg". Heute soll der Hund in der Tierklinik nur geimpft werden, doch das klingt einfacher, als es Rtl2now Prison Break. Da die kleine Hamsterdame etwa einen Tumor? Folge 15 noch keine Wertung. Tel: 22 70 E-Mail: post hedinger-praxis. Terminsprechstunde Um die Wartezeit für Sie so kurz wie möglich zu halten möchten wir Sie bitten einen Termin für Ihren Besuch zu vereinbaren. Tel: 20 30 Fax: 20 Jeffrey Dean Morgan Instagram E-Mail: jach tierarzt-jach. Folge 42 noch keine Wertung. Tel: 47 Fax: 53 E-Mail: Project X Stream Hd futter-vet. Weitere Informationen über Sozialfelle e. Terminsprechstunde - nach tel. Diagnostik akuter und chronischer Hauterkrankungen Zytologische und mikroskopische Untersuchungen. Ines Kussmann

Facebooktwitterredditpinterestlinkedinmail

3 Gedanken zu „Ines Kussmann“

  1. Ich entschuldige mich, aber meiner Meinung nach irren Sie sich. Es ich kann beweisen. Schreiben Sie mir in PM, wir werden reden.

    Antworten

Schreibe einen Kommentar